枯草芽胞杆菌YB-05对小麦抗病性相关防御酶系的诱导作用

本文研究了生防菌枯草芽胞杆菌YB-05和病原菌小麦全蚀病菌GGT007对小麦体内防御酶活性的影响,探讨其诱导小麦抗病性机理。以苯丙氨酸解氨酶(PAL)、过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)和多酚氧化酶(PPO)5种防御酶作为小麦抗病性反应指标,于不同时段测定各防御酶活性;以PD培养基为对照,测定生防菌YB-05及小麦全蚀病菌GGT007对小麦叶片和根部抗性相关酶的影响。结果表明,小麦经生防菌与病原菌混合处理、病原菌处理、生防菌处理后,叶片和根部与植物防御抗病相关的PPO、POD、SOD、PAL、CAT防御酶活性均比对照组高,其中生防菌与病原菌混合处理后抗性相关酶活最高,叶片中PAL、POD、SOD、PPO、CAT酶活峰值达到46.705、16 829.274、104.687、97.44和1 259.565U/g,为对照组的1.74、2.44、2.27、2.40和2.42倍。根部PAL、POD、SOD、PPO、CAT酶活峰值达到131.536、56 424.79、1 977.04、22.564和206.241U/g,为对照组的1.65、1.52、2.57、2.07、1.74倍。表明枯草芽胞杆菌YB-05和小麦全蚀病菌GGT007均能诱导小麦叶片和根部的防御酶活性增强,两者共同处理后小麦叶片和根部5种防御酶活性高于单独处理,说明枯草芽胞杆菌YB-05和小麦全蚀病菌GGT007共同诱导具有协同增效作用。

Induction of resistance-related defensive enzymes in wheat by Bacillus subtilis YB-05

The effects of Bacillus subtilis YB-05 and wheat take-all GGT007 on the activity of resistance-related defensive enzymes of wheat were investigated to analyze induced disease-resistant mechanism. Leaves and roots of wheat were treated with pathogen, biocontrol bacteria and their mixture, with PD medium as the control. The activities of 5 defensive enzymes in leaves and roots, including phenylalanine (PAL), catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and polyphenol oxidase (PPO), were indicated as disease-resistant indexes of wheat; the effects of YB-05 and GGT007 on 5 resistance-related defensive enzymes of wheat were studied at different stages. It was found that the PAL, POD, SOD, PPO and CAT activities in leaves and roots of the treated wheats were significantly higher than those in the control, and the activities in the treatment with pathogen mixed with biocontrol bacteria were the highest. The peak values of the PAL, POD, SOD, PPO and CAT activities of leaves were 46.705, 16 829.274, 104.687, 97.44 and 1 259.565 U/g, respectively, which were 1.74, 2.44, 2.27, 2.40 and 2.42 times as much as those of control group, respectively. And the peak values of the PAL, POD, SOD, PPO and CAT activities of roots were 131.536, 56 424.79, 1 977.04, 22.564 and 206.241 U/g, respectively, which were 1.65, 1.52, 2.57, 2.07 and 1.74 times as much as those of control group, respectively. It showed that B.subtilis YB-05 and wheat take-all GGT007 could increase the activities of resistance-related enzymes, so as to induce systemic resistance in wheat leaves and roots. The effects were greater when B.subtilis YB-05 and wheat take-all GGT007 were mixed than when treated alone, suggesting that they had a synergistic effect.