| 蔬菜作物根际促生菌分离筛选、鉴定及促生特性测定 |
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| 引用本文： | 李海云,蒋永梅,姚拓,侯栋,马亚春,张惠荣.蔬菜作物根际促生菌分离筛选、鉴定及促生特性测定[J].植物保护学报,2018,45(4):836-845. |
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| 作者姓名： | 李海云 蒋永梅 姚拓 侯栋 马亚春 张惠荣 |
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| 作者单位： | 甘肃农业大学草业学院草业生态系统教育部重点实验室;甘肃省农业科学院蔬菜研究所 |
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| 基金项目： | 国家自然科学基金（31660688），国家现代农业（大宗蔬菜）产业技术体系（CARS-23-G-19） |
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| 摘 要： | 为从4种蔬菜作物根际分离和筛选植物根际促生菌(plant growth promoting rhizobacteria,PGPR)并研究其促生特性,采用固氮、无机和有机磷培养基筛选根际促生菌株,对其促生特性进行定性和定量分析,并通过形态特征及16SrDNA序列分析对优良促生菌株进行鉴定。结果表明:从4种蔬菜根际中共获得57株PGPR菌株,其中固氮菌24株,溶磷菌33株。筛选出的19株优良PGPR菌株的固氮酶活性在0.14~3 664.97 nmol C2H4·h~(-1)·mL~(-1)之间,以NCRS1菌株固氮酶活性最高;溶解无机磷菌株的溶磷量在36.99~362.60μg/mL之间,培养液p H为4.57~5.75,以NCRP2菌株溶磷量最大;溶解有机磷菌株的溶磷量在9.24~55.21μg/mL之间,培养液p H为6.24~7.75,以PPRS3菌株溶磷量最大;菌株分泌IAA量均小于3.36μg/mL;NCRP2菌株对小麦长蠕孢病菌Helminthosporium tritici-vulgaris、番茄早疫病菌Alternaria solani、黄瓜枯萎病菌Fusarium oxysporum、马铃薯立枯丝核病菌Rhizoctonia solani、油菜菌核病菌Sclerotinias clerotiorum和玉米小斑病菌Bipolaria maydis均有抑制作用。对19株优良PGPR菌株进行初步鉴定,分属于假单胞菌属Pseudomonas、不动细菌属Acinetobacter、Advenella、叶杆菌属Phyllobacterium、细杆菌属Microbacterium、芽胞杆菌属Bacillus。本研究筛选出的优良PGPR菌株,可为今后生物菌肥推广应用提供菌种资源。
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| 关 键 词： | 植物根际促生菌 固氮菌 溶磷菌 促生机制 |
| 收稿时间： | 2017/6/16 0:00:00 |
Isolation, screening, identification and growth promoting characteristics of plant growth promoting rhizobacteria of vegetable crops |
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| Li Haiyun,Jiang Yongmei,Yao Tuo,Hou Dong,Ma Yachun and Zhang Huirong.Isolation, screening, identification and growth promoting characteristics of plant growth promoting rhizobacteria of vegetable crops[J].Acta Phytophylacica Sinica,2018,45(4):836-845. |
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| Authors: | Li Haiyun Jiang Yongmei Yao Tuo Hou Dong Ma Yachun and Zhang Huirong |
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| Institution: | Key Laboratory of Grassland Ecosystem, Pratacultural College, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China,Key Laboratory of Grassland Ecosystem, Pratacultural College, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China,Key Laboratory of Grassland Ecosystem, Pratacultural College, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China,Vegetable Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu Province, China,Key Laboratory of Grassland Ecosystem, Pratacultural College, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China and Key Laboratory of Grassland Ecosystem, Pratacultural College, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China |
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| Abstract: | |
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| Keywords: | plant growth promoting rhizobacteria (PGPR) nitrogen-fixing bacteria phosphate-solubilizing bacteria promoting mechanism |
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