| 应用PCR检测猪氟烷基因的研究 |
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| 引用本文： | 文雅芹,李爱云,曹国春,沈发云,连林生,唐爱发.应用PCR检测猪氟烷基因的研究[J].黑龙江畜牧兽医,2001,1(7):5-6. |
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| 作者姓名： | 文雅芹 李爱云 曹国春 沈发云 连林生 唐爱发 |
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| 作者单位： | [1]云南省原种猪场，云南昆明650212 [2]云南农业大学动科院，云南昆明650201 |
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| 摘 要： | 应用PCR－RFLP技术对云南省原种猪场核心群中88头引进外种猪（长白31头，大白59头）进行了氟烷基因的检测，结果检出9头杂合子，未发现阳性猪（nn）。研究表明：（1）长白猪群中不存在氟烷基因；（2）大白群中有9头杂合子且呈现全同胸或父系半同胞；（3）用猪毛进行氟烷基因检测具有方便、减少应激、准确性高等特点。
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| 关 键 词： | 氟烷基因 猪毛 PCR－RFLP 检测 应激综合症 |
| 文章编号： | 1004-7034(2001)07-0005-02 |
| 修稿时间： | 2000年12月8日 |
A Study on the Halothane Gene of Pigs Detected by PCR Technique |
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| WEN Yaqin ,LI Aiyun ,CAO Guochun ,SHEN Fayun ,LIAN Linsheng ,TANG Aifa.A Study on the Halothane Gene of Pigs Detected by PCR Technique[J].Heilongjiang Animal Science And veterinary Medicine,2001,1(7):5-6. |
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| Authors: | WEN Yaqin LI Aiyun CAO Guochun SHEN Fayun LIAN Linsheng TANG Aifa |
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| Institution: | WEN Yaqin 1,LI Aiyun 1,CAO Guochun 1,SHEN Fayun 1,LIAN Linsheng 2,TANG Aifa 2 |
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| Abstract: | The halothane gene in 88 alien bredding pigs including 31 Landrace and 57 Large White from foundational population in Primarybreeding farm in Yunnan Province was detected by PCR-RELPtechnique.The results showed:(1)halothane gene was not detected in Landrace group;(2) 9 halothane heterozygous pigs were detected in full-sib or half-sib of sire line from Large White group;(3)there areconvenience,reducing stress,high exactness and etc.merits in detectingof halothane gene using hog hair. |
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| Keywords: | Halothane Gene Hog Hair PCR-RFLP |
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