| 梓树属植物基因组DNA提取及RAPD体系优化 |
| |
| 引用本文： | 王念,王巧玲.梓树属植物基因组DNA提取及RAPD体系优化[J].河南林业科技,2012(1):1-3. |
| |
| 作者姓名： | 王念 王巧玲 |
| |
| 作者单位： | 1. 河南省林木良种选育与种质资源保护重点实验室,郑州,450008
2. 河南省林业调查规划院,郑州,450045 |
| |
| 基金项目： | 国家林业局“十二五”科技支撑项目（编号：2012BAD01805） |
| |
| 摘 要： | 以楸树幼嫩叶片为材料,进行楸树基因组DNA提取及RAPD扩增条件优化,试验表明,采用改良的2×CTAB法提取的DNA质量较高,适宜于RAPD分析。RAPD反应的优化体系为(25 ul反应体系):17.8 ul ddH2O;0.5 ul dNTP;2.5 ul 10×buffer;1.0 ul随机引物;2.0 ul Mgcl2;1.0 ul模版;0.2 ul Taq酶。
|
| 关 键 词： | 楸树 DNA提取 RAPD |
Genomic DNA Extraction and Optimization of RAPD System of Catalpa Scop |
| |
| WANG Nian,WANG Qiao-ling.Genomic DNA Extraction and Optimization of RAPD System of Catalpa Scop[J].Journal of Henan Forestry Science and Technology,2012(1):1-3. |
| |
| Authors: | WANG Nian WANG Qiao-ling |
| |
| Institution: | 1.Key Laboratory of Forest Germplasm Conservation. Breeding of Improved Variety of Henan-Province,Zhengzhou 450008, China; 2. Henan Forest Inventory and Planning Institute, Zhengzhou 450045, China) |
| |
| Abstract: | The method of genomic DNA extraction from new leaves and the optimization of RAPD analytic conditions were studies in Catalpa bungei. The result showed that the high-quality genomic DNA wasobtained by the revised 2 × CTAB method. The optimal PCR system for RAPD analysis was as foUows：17.6ul ddH2O;0.5ul dNTP;2.5ul 10× buffer; 1.01ul random Primer;2.0ul Mgcl2;1.0ul template;0.4ul Taq polymerase in 25ul reaction system. |
| |
| Keywords: | Catalpa bungei Genomic DNA extraction RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
