| 口蹄疫病毒Akesu/O/58株结构蛋白基因的克隆和细胞受体结合位点初探 |
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| 引用本文： | 王宝琴,张永光,潘丽,王文秀,吕建亮.口蹄疫病毒Akesu/O/58株结构蛋白基因的克隆和细胞受体结合位点初探[J].中国兽医杂志,2008,44(12). |
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| 作者姓名： | 王宝琴 张永光 潘丽 王文秀 吕建亮 |
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| 作者单位： | 1. 滨州学院生命科学系,山东,滨州,256600;中国农业科学院兰州兽医研究所,甘肃,兰州,730046
2. 中国农业科学院兰州兽医研究所,甘肃,兰州,730046 |
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| 基金项目： | 国家863高技术研究发展计划;口蹄疫可饲疫苗的研究
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甘肃省自然科学研究基金计划;口蹄疫植物反应器亚单位疫苗的研究
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| 摘 要： | 将Akesu/O/58口蹄疫病毒分离株牛舌皮毒适应乳鼠,通过RT-PCR法分别获得了该病毒结构蛋白基因vp1和p1.结果表明:vp1和p1基因分别为639 bp和2 208 bp,与Akesu/O/58细胞适应株FMDV的vp1和p1基因核苷酸序列的同源性分别为83.9%和84.7%,氨基酸序列的同源性为89.7%和95.1%.本试验分离株与OHK99、O1K、Taiwan97病毒株的细胞受体结合位点均为RGD(Arg-Gly-Asp),而Akesu/O/58细胞适应株的细胞受体结合位点为SGD(Ser-Gly-Asp).
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| 关 键 词： | 口蹄疫病毒 结构蛋白基因 克隆 细胞受体结合位点 |
Cloning of the structural protein gene and study of cell-recognition site of foot-and-mouth disease virus(Akesu/O/58) |
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| WANG Bao-qin,ZHANG Yong-guang,PAN Li,WANG Wen-xiu,LU Jian-liang.Cloning of the structural protein gene and study of cell-recognition site of foot-and-mouth disease virus(Akesu/O/58)[J].Chinese Journal of Veterinary Medicine,2008,44(12). |
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| Authors: | WANG Bao-qin ZHANG Yong-guang PAN Li WANG Wen-xiu LU Jian-liang |
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| Institution: | WANG Bao-qin1,2,ZHANG Yong-guang2,PAN Li2,WANG Wen-xiu2,LU Jian-liang2(1.Department of Life Sciences,Binzhou University,Binzhou 256503,China,2.Institute of Lanzhou Veterinary Medicine,Academy of Chinese Agriculture,Lanzhou 730046,China) |
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| Abstract: | The foot-and-mouth disease virus strain Akesu/58 serotype O from cattle epithelial blister was adapted for 3 serial passages in suckling mice.The total RNA of the virus was isolated and the structural protein genes vp1 and p1 were obtained by the RT-PCR procedures.These results showed that the sequence of vp1 and p1 gene contained 639 bp and 2 208 bp respectively.Compared with the sequence of the FMDV strain Akesu/58 serotype O published in GenBank,83.9% of vp1 and 84.7% of p1 nucleotide sequence identities... |
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| Keywords: | foot-and-mouth disease virus structural protein gene clone cell-recognition site |
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