| 延边牛线粒体DNA D-loop序列多态性研究 |
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| 引用本文： | 王朝锋,陈 宏,雷初朝.延边牛线粒体DNA D-loop序列多态性研究[J].西北农林科技大学学报(社会科学版),2005,33(7):9-11,16. |
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| 作者姓名： | 王朝锋 陈 宏 雷初朝 |
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| 作者单位： | 1. 西北农林科技大学,动物科技学院,陕西省农业分子生物学重点实验室,陕西,杨凌,712100
2. 西北农林科技大学,动物科技学院,陕西省农业分子生物学重点实验室,陕西,杨凌,712100;徐州师范大学,生物技术研究所,江苏,徐州,221116 |
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| 基金项目： | 国家“863”高技术研究发展计划项目(2003AA243051)，国家自然科学基金资助项目(30471238) |
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| 摘 要： | 对4头延边牛个体与GenBank中4头朝鲜牛个体的线粒体DNA(mtDNA)D环(D-loop区)910bp全序列进行了比较分析。结果发现,8头黄牛个体mtDNAD-loop序列由8种单倍型组成,单倍型比例为100%,表明2个黄牛品种mtDNA遗传多态性很丰富;在4头延边牛D-loop区序列中,共检测到核苷酸多态位点17个,核苷酸变异率为1.87%;在4头朝鲜牛中共检测到核苷酸多态位点10个,核苷酸变异率为1.10%;延边牛和朝鲜牛mtDNAD-loop全序列突变类型均为转换、颠换和插入/缺失,其中以转换为主;序列分析显示朝鲜牛与延边牛的同源性很高,达98.90%～99.67%,表明朝鲜牛和延边牛亲缘关系很近。
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| 关 键 词： | 延边牛 朝鲜牛 mtDNA D-loop 多态性 单倍型 |
| 文章编号： | 1671-9387(2005)07-0009-03 |
| 收稿时间： | 2004/10/15 0:00:00 |
| 修稿时间： | 2004年10月15 |
Study of mtDNA D-loop sequence polymorphism in Yanbian cattle |
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| WANG Chao-feng,CHEN Hong,LEI Chu-zhao,SUN Wei-bin,DANG Rui-hua,ZHANG Zhi-qing.Study of mtDNA D-loop sequence polymorphism in Yanbian cattle[J].Journal of Northwest Sci-Tech Univ of Agr and,2005,33(7):9-11,16. |
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| Authors: | WANG Chao-feng CHEN Hong LEI Chu-zhao SUN Wei-bin DANG Rui-hua ZHANG Zhi-qing |
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| Institution: | 1(1 College of Animal Science and Technology,Northwest A & F University,Shaanxi Key Laboratory ofAgricultural Molecular Biology,Yangling,Shaanxi 712100,China;2 Institute of Biotechnology,Xuzhou Normal University,Xuzhou,Jiangsu 221116,China) |
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| Abstract: | The complete mitochondrial D-loop sequences,910 bp in length,in 4 individuals from Yanbian cattle and 4 individuals from Korean cattle cited from GenBank were analyzed.The results showed that comparisons of these 8 sequences revealed 8 mitochondrial haplotypes and the percentage of haplotype was 100%,showing that abundant mitochondrial genetic diversity exists in Yanbian cattle and Korean cattle cattle breeds.Comparisons of these 4 sequences from Yanbian cattle revealed 17 polymorphic sites,with the percentage of 1.87% of 910 bp.Comparisons of the 4 sequences from Korean cattle revealed 10 polymorphic sites,with the percentage of 1.10% of 910 bp.And 3 types of mutation,transition,transversion /insertion were observed and transition was the major type of mutation.The percentage of homology of mtDNA D-loop was very high,up to 98.90%-99.67% between Yanbian cattle and Korean cattle,revealing close genetic relationship between Yanbian cattle and Korean cattle. |
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| Keywords: | Yanbian cattle Korean cattle mitochondrial DNA D-loop polymorphism haplotype |
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