| 天麻抗真菌蛋白cDNA克隆及植物表达载体的构建 |
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| 引用本文： | 李长福,张守鸿,葛正龙,范芳.天麻抗真菌蛋白cDNA克隆及植物表达载体的构建[J].贵州农业科学,2008,36(3):10-11. |
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| 作者姓名： | 李长福 张守鸿 葛正龙 范芳 |
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| 作者单位： | 遵义医学院生物化学与分子生物学教研室,贵州,遵义,563003 |
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| 摘 要： | 采用RT-PCR技术扩增出天麻抗真菌蛋白(Gastrodia antifungal protein,GAFP)的cDNA序列,通过pGM-T载体转入大肠杆菌JM109克隆,在培养基上筛选重组质粒酶切鉴定,阳性克隆经限制性内切酶Xba1和Sac1酶切与同样酶切的植物表达载体pBI121连接,构建天麻抗真菌蛋白(GAFP)的植物表达载体pBI121-GAFP。结果,天麻抗真菌蛋白(GAFP)的植物表达载体构建成功。
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| 关 键 词： | 天麻抗真菌蛋白 cDNA克隆 植物表达载体 |
| 文章编号： | 1001-3601(2008)03-0127-0010-02 |
| 修稿时间： | 2007年12月10 |
Gastrodia Antifungal Protein cDNA Clone and Construction of Plant Expression Vector |
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| LI Changfu,ZHANG Shouhong,GE Zhenglong,FAN Fang.Gastrodia Antifungal Protein cDNA Clone and Construction of Plant Expression Vector[J].Guizhou Agricultural Sciences,2008,36(3):10-11. |
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| Authors: | LI Changfu ZHANG Shouhong GE Zhenglong FAN Fang |
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| Abstract: | The cDNA nucleotide sequence of Gastrodia antifungal protein(GAFP) was amplified by RT-PCR and was directly transformed to E coli JM109 by a pGM-T vector.The recombinant plasmid was identified by the restriction enzyme on the media.The masculine clone cutted by restriction enzymes of Xba1 and the Sac 1 was successfully linked with plant expression vector pBI121,which constructs the plant expression vector of pBI121-GAFP. |
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| Keywords: | Gastrodia antifungal protein gene(GAFP) cDNA clone plant expression vector |
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