本地早橘的AFLP标记

采用AFLP技术分析了来自浙江黄岩的4个本地早橘材料(3个无核材料,1个有核材料),共筛选了72对E+3/M+3引物组合,从中选用12对多态性较好的引物进行最终扩增;最终得到9条清晰稳定的标记片断,分别命名为SL1～SL9;克隆测序后将所得碱基序列提交Genbank核酸序列数据库进行BLAST比对分析,结果表明,特异片段SL1与一种植物生长素输出蛋白编码基因PIN9有78%的一致性;特异片段SL2与一种植物反转录转座子基因有87%的一致性;特异片段SL4与植物叶绿体基因有94%的一致性;与功能基因较高的同源性为我们理解柑橘无核形成的分子机制提供了有价值的信息。

Study on Bendizao tangerine (Citrus succosa) by AFLP analysis

In this present research 4 Bendizao tangerine materials(3 seedless cultivars,1 seeded cultivar) were investigated by using AFLP technique.72 pairs of E 3/M 3 primer combinations were screened and 12 pairs of good polymorphism primers were selected for final PCR amplication.9 specific bands were generated and named as SL1 to SL9 respectively.These marker bands were cloned,sequenced and then the obtained sequence results were submitted to Genebank database with running BLASTn programme.The result showed that SL1 had 78% homology with putative auxin efflux carrier protein 9(PIN9) gene,SL2 had 87% homology with retrotransposon Rtsp-1 DNA sequence and SL4 had 94% homology with chloroplast genomic DNA sequence.The high homology with functional gene provided us valuable information about molecule mechanism of seedlees trait on citrus.