| 棉铃虫CYP4家族基因片段克隆及序列分析 |
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| 引用本文： | 艾颖,邱星辉,何凤琴.棉铃虫CYP4家族基因片段克隆及序列分析[J].河北农业大学学报,2004,27(6):60-64. |
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| 作者姓名： | 艾颖 邱星辉 何凤琴 |
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| 作者单位： | 中国科学院动物研究所,农业虫鼠害综合治理研究国家重点实验室,北京,100080 |
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| 基金项目： | "十五"国家科技攻关项目(2001BA509B08),中国科学院知识创新工程领域前沿项目(KSCX3-IOZ-04/05),国家自然科学基金资助(30370946) |
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| 摘 要： | 以5龄实验室品系棉铃虫的总RNA为模板,采用一对昆虫细胞色素P450简并引物,利用反转录—多聚酶链式反应(RT-PCR)扩增并筛选出10个新的长度约450bp的cDNA片段,并对这些序列与GeneBank中已知序列进行相似性分析,初步推测这10个P450基因片段属于CYP4家族的CYP4M和CYP4S2个亚家族。
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| 关 键 词： | 棉铃虫 细胞色素P450 反转录—多聚酶链式反应 基因克隆 |
| 文章编号： | 1000-1573(2004)06-0060-05 |
Cloning and sequence analysis of cytochrome P450 cDNA fragments of CYP4 family from Helicoverpa armigera |
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| AI Ying,QIU Xing-hui,HE Feng-qin.Cloning and sequence analysis of cytochrome P450 cDNA fragments of CYP4 family from Helicoverpa armigera[J].Journal of Agricultural University of Hebei,2004,27(6):60-64. |
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| Authors: | AI Ying QIU Xing-hui HE Feng-qin |
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| Abstract: | 10 new cDNA sequences encoding cytochrome P450 were amplified from the midgut cDNA library of the fifth instar larvae of a susceptible strains of the cotton bollworm, Helicoverpa armigera, by using a pair of degenerate primers designed according to the conservative amino acid sequences of CYP4 genes in insects by RT-PCR. The result of sequence analysis indicated that these 10 new cDNAs belong to CYP4M and CYP4S subfamilies. |
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| Keywords: | Helicoverpa armigera cytochrome P450 RT-PCR gene cloning |
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