抗菌肽Cecropin P1基因真核表达载体的构建

选择大肠杆菌偏爱密码子，人工设计合成3条抗菌肽Cecropin P1基因寡核苷酸片段，通过PCR扩增得到抗菌肽Cecropin P1基因的全序列，并将其克隆到T-easy载体上，经双酶切重组于真核表达质粒pcDNA3.1（+）上，经酶切鉴定和序列分析，抗菌肽Cecropin P1基因表达载体构建成功。抗菌肽Cecropin P1基因表达载体的成功构建，为进一步研究其抗菌活性、抗菌机理及应用等打下基础。

Construction of Eukaryotic Expression Vector of Antimicrobial Peptide Gene Cecropin P1

Three gene fragments were designed and synthesized according to the preference of E.coli.The gene of Cecropin P1 was amplified by PCR.The target gene fragment modified was cloned into vector T-easy,and then inserted into expression plasmid pcDNA3.1( ),the expression vector construction was completed.It was confirmed that the rombinant plasmid was successfully constructed by restriction endonuclease analysis and DNA sequencing,This may lay a foundation in research of antimicobial activities and antimicrobial mechanism in the gene of Cecropin P1.