谷蛋白和醇溶蛋白的电泳分析及小麦品种鉴定

应用不同浓度梯度的聚丙烯酰胺凝胺SDS电泳比较分析了18个小麦品种的、用不同提取方法制备的醇溶蛋白和谷蛋白.结果表明,经1M脲素提取掉多数醇溶蛋白、清蛋白及其它醇溶成分后的小麦样品,用加有2%SDS和5%β—巯基乙醇的裂解缓冲液提取的高分子量谷蛋白和醇溶蛋白,在8—12%梯度的SDS胶中显示出良好的分辨率和极大的多态性,适于小麦品种鉴定.用裂鲜缓冲液直接提取半粒小麦的全蛋白可用于逐粒品种鉴别.

THE ELECTROPHORETIC ANALYSIS OF GLUTENIN AND GLIADIN FOR WHEAT CULTIVAR IDENTIFICATION

A comparative electrophoretic analysis of gliadins and glutenins for 18 native Chinese cultivars extracted with different methods was made by using different gradient SDS polyacrylamide gel. The results reported here indicated that after removing most of gliadins, albumins and other alcohol - soluble substances by using 1M urea, those HMW ( high molecular weight ) glutenins and gliadins extracted with lysis buffer containing 2 % SDS and 5 % ?- mercaptoethanol showed a high resolution and considerable polymorphism in an 8-12% gradient polyacrylamide SDS gel. It is considered , therefore, this procedure may be useful in the cultivar identification of wheat. Besides, the total proteins extracted directly from half seed with lysis buffer may be useful for single seed characterization of wheat cultivars.