| 动物组织中四环素类抗生素残留的ELISA检测研究——土霉素抗体的制备 |
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| 引用本文： | 安清聪,张曦,陈克嶙. 动物组织中四环素类抗生素残留的ELISA检测研究——土霉素抗体的制备[J]. 畜牧与兽医, 2004, 36(9): 8-10 |
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| 作者姓名： | 安清聪 张曦 陈克嶙 |
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| 作者单位： | 云南农业大学动物科技学院,云南,昆明,650201 |
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| 摘 要： | 四环素类抗生素属小分子半抗原物质 ,必须与大分子物质结合才能产生免疫原性。本试验采用重氮化法合成土霉素 牛血清白蛋白 (BSA OTC)及土霉素 鼠血清白蛋白 (RSA OTC)人工抗原 ,用紫外扫描对其进行鉴定。以BSA OTC免疫家兔制备抗血清 ,以RSA OTC为包被抗原 ,采用双向免疫扩散法和ELISA方法对抗血清进行鉴定。结果表明用重氮化法合成的人工抗原对四环素类药物有特异的抗原抗体反应 ,抗血清效价达 1 2 8× 1 0 5,完全能够满足四环素类药物残留的ELISA检测要求
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| 关 键 词： | 合成抗原 抗体 ELISA |
| 文章编号： | 0529-5130(2004)09-0008-03 |
Detection of tetracyclines residue in animal tissues by ELISA:preparation of anti-OTC antibodies |
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| AN Qin-cong,ZHANG Xi,CHEN Ke-ling. Detection of tetracyclines residue in animal tissues by ELISA:preparation of anti-OTC antibodies[J]. Animal Husbandry & Veterinary Medicine, 2004, 36(9): 8-10 |
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| Authors: | AN Qin-cong ZHANG Xi CHEN Ke-ling |
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| Abstract: | OTC-conjugated antigen (BSA-OTC or RSA-OTC) produced by coupling diazotized OTC with bovine sera albumin (BSA) or rat sera albumin (RSA) was successfully identified by ultraviolet spectrophotometer. The rabbits were immunized with BSA-OTC to produce antibodies which were identified by indirect enzyme-linked immunosorbent assay (ELISA) using RSA-OTC. The results showed that high titers of 1.28×10~5 anti-OTC serum were produced by BSA-OTC immunized rabbits. It provided a necessary condition for ELISA detecting tetracycline antibiotic (oxytetracycline, tetracycline and aureomycin) residues. |
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| Keywords: | OTC-conjugated antigen antibodies ELISA |
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