六倍体小黑麦萌发期抗旱性分析

利用20%PEG-6000(-0.975 MPa)为渗透介质室内模拟干旱,分析六倍体小黑麦萌发期发芽率、发芽势、胚芽鞘长、根长、根数的变化,采用模糊隶属函数与抗旱系数相结合的方法对品种萌发期的抗旱性进行综合分析,并利用灰色关联分析法分析各个形态指标与抗旱性的关系,结果表明:干旱胁迫下,小黑麦各品种的发芽率、发芽势、胚芽鞘长、根长都比对照不同程度地降低或缩短,不同品种之间的差异达显著或极显著水平,但根数却增减不一,表明根数对水分胁迫的反应方向不一致;品种"Tornado"(S9)萌发期综合抗旱性强,为小麦抗旱育种提供了种质资源;发芽率与六倍体小黑麦萌发期抗旱性的关联度最大,可作为形态指标加以利用。

Analysis of drought resistance of hexaploid triticale at sprouting stage

20% PEG-6000(-0.975 MPa) was used to induce water stress in the laboratory to determine the germination potential,germination rate,coleoptile length,radicle length and root number of hexaploid triticale at sprouting stage.Drought resistance of hexaploid triticale was analyzed by fuzzy subordinate function analysis and drought-resistance coefficient at sprouting stage.Finally,the relationship between each morphological index and drought resistance was established using grey correlative degree analysis.Results show that the germination potential,germination rate,coleoptile and radicle length of each variety decrease or shorten than those of the control under osmotic stress.Differences among triticale varieties are either significant or very significant,but root numbers of some triticale varieties increase while others decrease,indicating that reactions of root number to water stress are different.The variety "Tornado"(S9) has high resistance to drought at sprouting and can be used as a good germsplasm in wheat breeding.The degree of correlation between germination rate and drought resistance of hexaploid triticale at sprouting stage is simply highest,hence germination rate can be a useful morphological index of drought resistance.