An improved hemagglutination test for study of canine parvovirus |
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| Authors: | M Senda N Hirayama H Yamamoto K Kurata |
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| Institution: | 1. First Viral Disease Section, National Veterinary Assay Laboratory, Kokubunji, Tokyo 185, Japan;2. Technical Extension Service Division, Nippon Institute for Biological Science, Ome, Tokyo 198, Japan;1. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;2. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310058, China;3. Yunnan Provincial Center of Arborvirus Research, Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research,Yunnan Institute of Parasitic Diseases, Pu’er, Yunnan 665000, China;4. Yunnan Institute of Endemic Disease Control and Prevention, Yunnan Provincial Center of Virus and Rickettsia Research, Dali, Yunnan 671000, China;1. School of Public Health, Anhui Medical University, Hefei, 230032, PR China;2. College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China;3. Municipal Key Laboratory of Virology, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, PR China;4. Hefei Minghang Breeding Co. Ltd, Hefei, 231262, PR China;1. Laboratory of Small Animal Clinics, Veterinary Teaching Hospital, Azabu University, 1-17-71, Fuchinobe, Chuo, Sagamihara 252-5201, Kanagawa, Japan;2. Laboratory of Pathology, Department of Environmental Hygine, Azabu University, 1-17-71, Fuchinobe, Chuo, Sagamihara 252-5201, Kanagawa, Japan;3. Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu 183-8509, Tokyo, Japan;4. Research Institute of Biosciences, Azabu University, 1-17-71, Fuchinobe, Chuo, Sagamihara 252-5201, Kanagawa, Japan |
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| Abstract: | Optimal conditions for hemagglutination (HA) by canine parvovirus (CPV) strains were investigated using several buffers. Porcine erythrocytes often agglutinated spontaneously in phosphate-buffered salt solution, isotonic saline solution or barbitone-complement-fixation buffer. Results were reproducible when borate-buffered saline (BBS) was used as the diluent for antigen, and "virus adjusting diluent" (VAD), containing 0.15 M NaCl and 0.3 M phosphate was used as the diluent for erythrocytes. Highest HA titers were obtained at pH 6.0 using BBS and VAD. Specific HA with CPV was observed not only at 4 degrees C but at 37 degrees C, and erythrocytes from horse, shrew mouse, hamster, cat, sheep and dog, as well as pig and African green monkey were agglutinated by CPV using the improved method. |
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