| 云南出口新鲜松茸产地属性DNA指纹图谱构建 |
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| 引用本文： | 陈丽萍,;杨玲春,;陈芸,;陈旭,;岳亮亮,;刘忠民,;傅士华,;郭永红. 云南出口新鲜松茸产地属性DNA指纹图谱构建[J]. 中国食用菌, 2014, 0(5): 43-50 |
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| 作者姓名： | 陈丽萍, 杨玲春, 陈芸, 陈旭, 岳亮亮, 刘忠民, 傅士华, 郭永红 |
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| 作者单位： | [1]云南出入境检验检疫局,云南昆明650228; [2]中华全国供销合作总社昆明食用菌研究所,云南昆明650221 |
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| 基金项目： | 国家质量监督检验检疫总局科技计划项目“松茸产地属性DNA指纹图谱库的建立”（2011IK246）;“十一五”国家科技支撑计划课题“食用菌质量安全与标准化技术研究”（2008BADAIB08）. |
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| 摘 要： | 收集19个出口松茸主产地的97个样本,使用逆转录因子的DNA分子标签对样本基因组进行PCR扩增;为尽可能区分原产地,对某些产地的样本增加先使用限制性内切酶处理基因组DNA,再次使用逆转录分子标签的步骤。采用全自动DNA分析系统获得一系列DNA指纹图谱。通过比较分析找到同一地理居群共有的DNA指纹图谱特征以及酶切前后的图谱差异。这些指纹图谱特征与松茸原产地间存在显著相关性。
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| 关 键 词： | 云南出口松茸主产地 限制性内切酶 全自动DNA分析系统 |
The Establishment of DNA Finger Print Illustrations of Yunnan T. matsutake for Commercial Export |
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| Affiliation: | CHEN Li-ping, YANG Ling-chun, CHEN Yun, CHEN Xu, YUE Liang-liang, LIU Zhong-min, FU Shi-hua, GUO Yong-hong ( 1. Yunnan Entry - Exit Inspection and Quarantine Bureau, Kunming Yunnan 650228 ; 2. Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooperatives, Kunming Yunnan 650221 ) |
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| Abstract: | 97 T. rnatsutake samples for commercial exports were obtained from 19 geographical origins. A set of DNA markers tar- geting retroelement integration sites of those T. matsutake samples were PCR - amplified to generate DNA amplification polymor- phism. As far as possible to differentiate among T. matsutake samples based on their geographical origins, some particular sam- ples genomic DNA need to be treated with restriction endonuelease digestion, then were amplified by DNA markers again. An au- tomatic DNA analysis system was used to generate a set of DNA finger print illustrations. Through the comparative analysis, com- mon DNA fingerprint characteristics of same geographical populations and differences between before and after restriction endonu- elease digestion could be recognized. It was inferred that a significant correlation between the fingerprint characteristics and geo- graphical origin of T. matsutake. |
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| Keywords: | Geographical origins Restriction endonuclease Automatic DNA analysis |
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