纳米级小肽螯合铜对小白鼠铜排泄和免疫指标的影响

试验的目的是考查以硫酸铜、碱式氯化铜、蛋氨酸铜、纳米铜和纳米小肽螯合铜等不同铜源供给形式对小白鼠铜排泄和血液免疫指标的影响。试验将120只18日龄小白鼠随机分为Ⅰ(小肽+硫酸铜组)、Ⅱ(硫酸铜组)、Ⅲ(碱式氯化铜组)、Ⅳ(蛋氨酸铜组)、Ⅴ(纳米铜组)和Ⅵ(纳米小肽螯合铜)等6个处理组,每个处理组设4个重复,每个重复5只小白鼠。饲喂试验分0～56、～121、3～192、0～26和27～33 d 5个阶段。其中0～5 d为预饲期,后4个阶段为试验期。预饲期和试验期各处理组采用相同的基础日粮。试验期Ⅰ～Ⅵ组饲粮铜的含量(以铜计)在6～12、13～19、20～26和27～33 d 4个阶段分别为2.5、5、10及15 mg/kg。结果表明,纳米小肽螯合铜组小鼠的铜排泄量显著低于其它各组。饲喂第2、3、4阶段,纳米小肽螯合铜组小鼠的血清免疫蛋白IgGI、gMI、gA、胰岛素样生长因子-1(IGF-1)及白介素-2(IL-2)均极显著(P<0.01)高于其它各组。

Effect of nano copper-small peptide chelate on excretion of copper and immune indexes of mice

The purpose of this study was to examine influence of the different copper sources include copper sulfate,basic copper chloride,copper-methionine,nano-copper and nano copper-small peptide chelate on copper excretion and key immune indexes of mice.120 18-day-old mice were randomly divided into six treatment groups,Ⅰ(group of small peptide + copper sulfate),Ⅱ(copper sulfate group),Ⅲ(basic copper chloride group),Ⅳ(copper methionine),Ⅴ(nano-copper group) and Ⅵ(nano copper-small peptide chelate group),each treatment group with four duplicates,and each duplicate with five mice.Feeding trial was devided in to five phases from 0~5,6~12,13~19,20~26 and 27~33 d.The 0~5 d phase is the pre-trial period,and all the groups used the same basic diet.In the five trial phases,dietary copper content were supplied with 5,2.5,5,10 and 15 mg per kg feed separately.The results showed that the copper excretion of nano copper-small peptide chelate group is obviously lower than that of other groups(P<0.05).During the feeding phases of 2,3 and 4,the levels of IgG,IgM,IgA,IGF-1 and IL-2 in blood serum of mice in the group Ⅵ were notablely higher than that of other groups(P<0.01).