胶东地区耐药猪源大肠杆菌Ⅰ型整合子-基因盒流行状况分析

在胶东地区119株多重耐药大肠杆菌中,共92株菌有I型整合子整合酶基因,检出率达77.31%。92株I型整合子携带大肠杆菌共扩增出7类不同大小的基因盒插入区片段,大小分别为1008bp、1318bp、1586bp、1663bp、1913bp、1945bp、3149bp,其中以大小为1008bp的插入区片段的检出率最高,为31.67%。而检出率最低的为长度为1318bp的基因盒插入区。将基因盒插入区扩增片段的测序结果与Genebank中的相关序列进行比对,得出Ⅰ型整合子携带的耐药基因盒种类分别为aadA1、aadA2、aadA5、aadA22、aadB、dfrA1、dfrA12、dfrA17、dfrA27、lnuF、cmLA6、aar-3、orfF分别编码对相应药物的耐药性。

Analysis of the Prevalence of Class I Integron and its Gene Cassette in the Multiple Resistant E.coli Strains Collected from Swine Farms in Jiaodong

92 out of 119 multiple resistant E.coli strains from swine farms in Jiaodong possessed class I integrou gene (77.31%).In the 92 isolates carrying class I integrous there existed 7 kinds of gene cassette integron segments with 1008bp、1318bp、1586bp、1663bp、1913bp、1945bp、3149bp in length respectively.1008bp segment was mostly detected upto 31.67%,and the 1318bp segment was least detected,onle 1.67%.Comparison of the sequencing results of gene cassette with the correspinding sequences in Genebank revealed that the gene cassettes encoding the antibiotic resistence were aadA1、aadA2、aadA5、aadA22、aadB、dfrA1、dfrA12、dfrA17、dfrA27、lnuF、cmLA6、 aar-3、orfF.