超声波辅助提取杠柳脂溶性成分工艺优化

为了优化超声波辅助提取杠柳脂溶性成分的工艺,该文在单因素试验基础上,选取超声波功率、提取时间、液料比为主要影响因素,分析了3个主要影响因素对杠柳脂溶性成分提取结果的影响,应用响应面分析法优化了超声波辅助提取杠柳脂溶性成分的工艺方法,并使用GC-MS联用仪检测了杠柳脂溶性提取物的主要成分。结果表明:超声波辅助提取杠柳营养体脂溶性成分的最佳工艺为:在室温(20℃)及超声波频率20kHz条件下,提取试剂为石油醚,超声波辅助提取时间为70min,超声波功率为730W,料液比为1∶10g/mL。该工艺条件下,杠柳脂溶性成分的实际提取得率可达到6.14%。

Optimization of ultrasonic-assisted extraction technology of fat-soluble components from Periploca sepium bunge

For optimizing the ultrasound-assisted extraction technology of fat-soluble components from Periploca sepium Bunge, on the basis of single-factor experiments, ultrasonic power, solid-solvent ratio and ultrasonic time were selected as main influencing factors to analysis the effects on extraction results of fat-soluble components from Periploca sepium Bung by response surface analysis methodology. The main fat-soluble components extracted from Periploca sepium Bunge were detected by using GC-MS. The results showed that the optimum conditions for ultrasound-assisted extraction of fat-soluble components from Periploca sepium Bunge were as follow: under the room temperature of 20℃ and ultrasonic frequency of 20 kHz with petroleum ether as chemical reagent, ultrasonic assisted extraction time 70 min, ultrasonic power 730 W, material-solvent ratio 1:10 g/mL. Under these conditions, the actual extraction yield of fat-soluble components from Periploca sepium Bunge reached to 6.14%.