| 蜘蛛拖牵丝蛋白基因的克隆与表达 |
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| 引用本文： | 刘丹梅,王芬,李文利.蜘蛛拖牵丝蛋白基因的克隆与表达[J].广西农业生物科学,2011,30(1):16-20. |
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| 作者姓名： | 刘丹梅 王芬 李文利 |
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| 作者单位： | 大连理工大学环境与生命学院,大连,116023 |
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| 摘 要： | 蜘蛛丝具有极高的强度和韧度,工业和医学应用价值很高,但由于蜘蛛的不可驯养性使其应用受到限制。因此,本文尝试利用基因工程的方法获得蛛丝蛋白的表达。我们利用巢式PCR技术从大腹圆蛛Araneus ventricosus基因组中克隆了长度为837bp的拖牵丝蛋白基因（ASP）,并分别将其构建至原核表达载体pGEX-6p-1和真核表达载体pGFP-N2上,分别命名为pASG和pASN。pASG在大肠杆菌中16℃下24h诱导表达后,经蛋白质印迹证明成功地表达了GST-ASP融合蛋白;pASN转染昆虫sf9细胞48h后观察到了绿色荧光蛋白GFP的表达,表明ASP基因在大肠杆菌和真核细胞中分别得到了正确表达。本研究为利用基因工程的方法开发蛛丝蛋白的生产途径提供了有益的尝试。
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| 关 键 词： | 拖牵丝蛋白基因 基因克隆 原核表达 真核表达 |
Cloning and Expression of Spider Dragline Silk Protein Gene in Escherichia coli and Eukaryotic Cells |
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| Liu Danmei , Wang Fen Li Wenli School of Environmental & Biological Science & Technology,Dalian University of Technology,Dalian, School of Agriculture,Eastern Liaoning University,D,ong.Cloning and Expression of Spider Dragline Silk Protein Gene in Escherichia coli and Eukaryotic Cells[J].Journal of Guangxi Agricultural and Biological Science,2011,30(1):16-20. |
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| Authors: | Liu Danmei Wang Fen Li Wenli School of Environmental & Biological Science & Technology Dalian University of Technology Dalian School of Agriculture Eastern Liaoning University D ong |
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| Institution: | Liu Danmei 1,2 Wang Fen 1 Li Wenli 1* 1 School of Environmental & Biological Science & Technology,Dalian University of Technology,Dalian,116023,2 School of Agriculture,Eastern Liaoning University,Dandong,118001 |
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| Abstract: | The extreme tensile strength and toughness of spider silk make it a superior candidate for a wide range of medical and industrial applications. In this study, a 837 bp fragment which encode dragline silk gene （ASP） was cloned from the genome of spider （Ar |
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| Keywords: | Dragline silk gene Gene cloning Prokaryotic expression Eukaryotic expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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