几株高效溶磷菌株对不同磷源溶磷活力的比较

在液体培养条件下，研究了4株溶磷菌株(Bmp5、Bmp6、Bmp7和Fmp9)对不同磷源溶解能力的差异并与荧光假单孢菌As1.867和巨大芽孢杆菌As1.223进行了比较，探讨了菌株组合培养对溶磷活力的影响。结果表明，4株菌株对磷酸钙、磷酸铝、磷酸氢钙溶解能力明显高于磷酸铁和卵磷脂。以磷酸钙为磷源时，Fmp9的溶磷量比As1.867和As1.223分别高出约92%和48%；而以磷酸铝为磷源时，As1.223的溶磷量明显高于其他菌株；在磷酸氢钙为磷源的条件下，Bmp6为优势菌株，溶磷量高达785.51mg/L。对比研究发现，Bmp5、Bmp6、Bmp7及Fmp9的优势磷源分别为卵磷脂、磷酸氢钙、磷酸铝和磷酸钙。组合培养表明，Bmp5+Fmp9和Bmp6+Fmp9较单株菌的溶磷量有所增加，为较好的组合。试验得到的溶磷微生物配方已经应用于生物复合肥料的研究，并进行了盆栽实验，得到了较好的效果。该研究可为土壤生物肥料工业的微生物学研究提供借鉴。

Comparative research on the influences of several high efficient phosphate-solubilizing strains on phosphate solubilizing activity

Under hydroponic conditions, the phosphate solubilizing capacity of the four tested strains(Bmp5, Bmp6, Bmp7 and Fmp9) in dissolving five different poorly soluble phosphates were studied with two strains(Pesudomonas fluorescens As1.867 and Bacillus megatherium As1.223) as control. A co-culture experiment with these four strains, compared to the single cultivation, was conducted in order to reveal their mutual influences on the phosphate solubilizing activity. The results indicated that these single strains exhibited higher solubilizing abilities in Ca₃(PO₄)₂, AlPO₄·2H₂O and CaHPO₄·2H₂O than in FePO₄·2H₂O and lecithin. As an example, when using Ca₃(PO₄)₂ as the sole phosphate source, the amount of the phosphate solubilizing capacity of Fmp9 was 92% and 48% higher than those of the references As1.867 and As1.223, respectively. As1.223 showed the maximum solubilization in AlPO₄·2H₂O. In all four tested strains, Bmp6 was the dominant strain in solubilizing CaHPO₄·2H₂O, and its phosphate solubilization capacity could be as high as 785.51 mg/L. The best phosphate sources for Bmp5, Bmp6, Bmp7 and Fmp9 were lecithin, CaHPO₄·2H₂O, AlPO₄·2H₂O and Ca₃(PO₄)₂. Furthermore, the study of the hydroponic co-culture of the four strains showed that the combinations of Bmp5+Fmp9 and Bmp6+Fmp9 were the preferable combinations in terms of phosphate solubilizing activities. The phosphate solubilizing microorganisms in this experiment have been used to the biological compound fertilizer and the pot experiment showed a satisfactory result. This research can provide referencesfor biological compound fertilizer.