高产嗜铁素恶臭假单胞菌A3菌株的鉴定及其对黄瓜的促生作用

从大棚蔬菜根际土中分离到一株嗜铁素高产菌株A3，铬天青（CAS）法定量检测其嗜铁素相对含量达93.40%，Shenkers实验确定为羧酸型嗜铁素。在不同底物诱导下，该菌株可不同程度地产生吲哚乙酸（IAA）及1-氨基环丙烷-1-羧酸（ACC）脱氨酶，并具有一定的溶磷能力。根据形态特征、生理生化、API系统及16S rRNA基因序列分析，将菌株A3鉴定为恶臭假单胞菌（Pseudomonas putida）。在缺铁Hoagland营养液中添加难溶性铁及菌株A3嗜铁素发酵滤液的处理组，能够显著提高黄瓜幼苗的株高、根长、叶长、鲜重及叶绿素含量，表明菌株A3产生的嗜铁素在低铁条件下对黄瓜幼苗具有促生作用。

Identification of a siderophore-producing bacterium Pseudomonas putida A3 and its growth-promoting effects on cucumber seedlings

Bacterial strain A3 with high ability of producing siderophore was isolated from rhizosphere of greenhouse vegetables.The relative siderophore content was measured by chrome azurol S(CAS) assay and was identified using the Shenker's test.The results show that the relative siderophore content is 93.40% and the type is a kind of carboxylate type siderophore.In addition,the strain A3 also has a high ability of solubilizing phosphate and is able to produce indol-3-acetic(IAA) and 1-aminocyclopropane-1-carboxyla...