| 宜昌百合体细胞胚诱导及植株再生 |
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| 引用本文： | 孙安妮,张延龙,牛立新,王仙芝,崔雅静,王润丰.宜昌百合体细胞胚诱导及植株再生[J].西北农业学报,2011,20(8):142-146. |
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| 作者姓名： | 孙安妮 张延龙 牛立新 王仙芝 崔雅静 王润丰 |
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| 作者单位： | 西北农林科技大学林学院,陕西杨凌,712100 |
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| 基金项目： | 国家林业总局重点攻关项目(2006-73) |
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| 摘 要： | 为了对秦岭野生宜昌百合资源进行保存,并有效地解决其扩繁和再生问题,以秦岭野生宜昌百合为材料,研究不同质量浓度的激素组合、鳞片的不同部位及光暗培养对秦岭野生宜昌百合的体细胞胚诱导的影响。结果表明,鳞片诱导体细胞胚的适宜培养基为MS+0.5 mg/L TDZ(N-苯基-N-′1,2,3-噻二唑-5-脲)+1.0 mg/L NAA(a-萘乙酸)。鳞片基部诱导体胚率最高,为90.00%,鳞片分化体细胞胚的能力为基部>中部>上部。暗培养下诱导率为90.00%,高于光培养条件。
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| 关 键 词： | 秦岭野生宜昌百合 鳞片 诱导 体细胞胚 |
Somatic Embryogenesis and Plantlet Regeneration of Lilium leucanthum |
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| SUN Anni,ZHANG Yanlong,NIU Lixin,WANG Xianzhi,CUI Yajing and WANG Runfeng.Somatic Embryogenesis and Plantlet Regeneration of Lilium leucanthum[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(8):142-146. |
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| Authors: | SUN Anni ZHANG Yanlong NIU Lixin WANG Xianzhi CUI Yajing and WANG Runfeng |
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| Institution: | SUN Anni,ZHANG Yanlong,NIU Lixin,WANG Xianzhi,CUI Yajing and WANG Runfeng(College of Forestry,Northwest A&F University,Yangling Shaanxi 712100,China) |
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| Abstract: | The objective of this paper was to preserve and propagate the wild resources and to establish a simplest and efficient method for high frequency of somatic embryogenesis and plant regeneration of Lilium leucanthum(Baker).The effects of hormone combination,different places in scales and the condition of light or dark on inducing somatic embryogenesis were studied.The results showed that the appropriate medium of the somatic embryogenesis induced from scales was MS + 0.5 mg/L TDZ + 1.0 mg/L NAA.The induction ... |
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| Keywords: | Wild Qinling Yichang lily Scale Induction Somatic embryogenesis |
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