苹果砧木组织培养与快繁技术研究

通过对不同苹果砧木M9、M26、八棱海棠、平邑甜茶的离体培养研究,探讨不同砧木的初代、继代培养、生根以及移栽技术.结果表明,①以4种砧木的离体新梢进行初代培养,最佳培养基均为MS+ 6-BA1.0mg·L1+NAA 0.1mg· L-1.②M9与M26的最佳增殖培养基MS+ 6-BA 1.0 mg· L-1+IBA 0...

Studies on Tissue Culture and Rapid Propagation of Apple Stocks

In this paper,techniques about primary culture,subculture,root reducing and transplant in different apple stocks were discussed by studying in vitro culture of four apple rootstock breeds,including M-9,M-{26},Malus micromalus Mark.,Malus hupenensis Rehd.The results indicated that,① For all this four apple rootstocks,the medium of MS+6-BA1.0 mg·L{-1}+NAA0.1 mg·L{-1} is the best when their shoots cultivated in vitro.② For M-9 and M-{26},the optimum enrichment medium and the optimum rooting medium were MS+6-BA 1.0 mg·L{-1} +IBA 0.1 mg·L{-1} and 1/2MS+IBA{0.3} {mg·L}{-1} +NAA0.1 mg·L{-1},respectively.The rooting rates were 70.6% and 69.6%,respectively.③To Malus micromalus Mark and Malus hupenensis Rehd,the optimum subculture medium and the optimum rooting medium were MS+6-BA1.0 mg·L{-1}+IBA0.3 {mg·L}{-1} and 1/2MS+IBA0.3 mg·L{-1},respectively.Both of the rooting rates were over 80%.④ The nursery substrate treatment with 0.5 mg·L{-1} FeSO-4 made the survive rate of the root regeneration plant above 90%.