| 宁夏枸杞中蛋白质的提取与鉴定 |
| |
| 引用本文： | 李一婧,马伟超,王廷璞,袁毅君,梁芳梅. 宁夏枸杞中蛋白质的提取与鉴定[J]. 种子, 2011, 30(9) |
| |
| 作者姓名： | 李一婧 马伟超 王廷璞 袁毅君 梁芳梅 |
| |
| 作者单位： | 天水师范学院生命科学与化学学院,甘肃天水,741000 |
| |
| 基金项目： | 2008人社部留学人员项目; 天水师范学院科研项目(TSB0506) |
| |
| 摘 要： | 采用盐溶法提取宁夏枸杞中蛋白质,用饱和(NH4)2 SO4溶液进行分级盐析、透析、浓缩后用SDS-PAGE分析,测定不同提取液中主要蛋白质的分子量.结果表明:用pH为6.0的PB缓冲液提取,用饱和度为80％的(NH4)2SO4盐析时,枸杞蛋白的提取效果最好；经SDS-PAGE电泳后,初步鉴定出7种主要蛋白质,其中分子量为63 096 Da、51 286 Da、33 884Da的蛋白质提取范围较大.
|
| 关 键 词： | 枸杞 蛋白质 提取 鉴定 |
Extraction and Identification Protein of Lycium barbarum L.in Ningxia |
| |
| LI Yi-jing,MA Wei-chao,WANG Ting-pu,YUAN Yi-jun,LIANG Fang-mei. Extraction and Identification Protein of Lycium barbarum L.in Ningxia[J]. Seed, 2011, 30(9) |
| |
| Authors: | LI Yi-jing MA Wei-chao WANG Ting-pu YUAN Yi-jun LIANG Fang-mei |
| |
| Affiliation: | LI Yi-jing,MA Wei-chao,WANG Ting-pu,YUAN Yi-jun,LIANG Fang-mei(College of Bioscience and Chemistry,Tianshui Normal University,Tianshui Gansu 741001,China) |
| |
| Abstract: | The protein of Lycium barbarum L.in Ningxia were extracted by the salt solvent method,and purified by ammonium sulfate fractionation,dialysis,concentration and the molecular weights of the proteins from different extraction solution were measured by SDS-PAGE analysis.The result showed that the optimum Lycium barbarum L.protein extraction condition was pH 6.0 PB Buffer,80% ammonium sulfate.The SDS-PAGE analysis results showed that 7 kinds of protein were identified,and 3 kinds of protein with molecular weigh... |
| |
| Keywords: | Lycium barbarum L. proteins isolation identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
