| 雪莲组培过程对潮霉素敏感性的研究 |
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| 引用本文： | 唐亚萍,覃建兵,原慧. 雪莲组培过程对潮霉素敏感性的研究[J]. 种子, 2011, 30(9) |
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| 作者姓名： | 唐亚萍 覃建兵 原慧 |
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| 作者单位： | 新疆师范大学分子生物与生物信息研究室,乌鲁木齐,830053 |
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| 基金项目： | 科技部援疆计划项目(200840102-39); 新疆师范大学研究生科技创新基金项目(20101211) |
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| 摘 要： | 以雪莲叶片为外植体,研究了不同浓度的潮霉素对雪莲愈伤组织诱导、生长及分化的影响,结果表明:8.0 mg/L浓度的潮霉素为雪莲叶盘转化筛选的最佳质量浓度,不同浓度(5 ～50 mg/L)潮霉素对MS+ NAA 2.00 mg/L+ 6-BA 1.00mg/L培养基下雪莲愈伤组织的诱导与生长均有明显的抑制作用,随着潮霉素浓度的升高,毒害程度加深,当潮霉素浓度为20 mg/L时表现为生长的愈伤组织块较小、褐化、甚至死亡.同时,低浓度(0.5 ～2.0 mg/L)的潮霉素可以提高雪莲愈伤组织的分化率,其中0.5 mg/L潮霉素的质量浓度的诱导培养基内,雪莲愈伤组织的出芽率平均可达98.33％.
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| 关 键 词： | 雪莲 愈伤组织 潮霉素 |
Study on the Sensitivity of Saussurea involucrate to Hygromycin During Tissue Culture |
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| TANG Ya-ping,QIN Jian-bin,YUAN Hui. Study on the Sensitivity of Saussurea involucrate to Hygromycin During Tissue Culture[J]. Seed, 2011, 30(9) |
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| Authors: | TANG Ya-ping QIN Jian-bin YUAN Hui |
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| Affiliation: | TANG Ya-ping,QIN Jian-bin,YUAN Hui(Research Room of Molecular Biology and Informatics,Xinjiang Normal University,Urumqi 830053,China) |
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| Abstract: | To study the effects on induction,growth and differentiation of callus(Saussurea involucrate),the hygromycin and Saussurea involucrate leaves were used as stress and explants,respectively.Cultured on MS+ NAA 2.00 mg/L+6-BA 1.00 mg/L media added different concentration(5 mg/L to 50 mg/L) of hygromycin,the result showed that hygromycin of 8 mg/L was the best concentration for screening calli,there were significantly inhibition on callus induction and growth,and the damagement increased with the concerntration... |
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| Keywords: | Saussurea involucrate callus hygromycin |
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