史氏鲟精子超低温冷冻保存研究

比较了史氏鲟精子在3种不同配比浓度稀释液的保存效果。试验结果表明,配方Ⅲ作为稀释液,8%甲醇作为抗冻剂,二步法超低温(-196℃)冷冻保存,5h后取出,38℃水浴解冻取得最好的冻后激活率,解冻后激活率为(52.3±3.5)%。解冻精子分别采用井水和激活液D(10mmol/L Tris+10mmol/L NaCl+25mmol/L Glu,pH 8.0)激活,进行人工授精。结果显示配方Ⅲ冻精采用激活液D激活授精获得最高受精率为68.56%,最高孵化率为52.91%。本次试验表明,1～2mmol/L范围内,低浓度K+比高浓度K+对史氏鲟精子保存有利;52～82mosmol/kg范围内,高渗稀释液有利于史氏鲟精子的保存;且激活授精方法是影响冻精受精率和孵化率的关键因素之一。

Cryopreservation of Sperm in Amur Sturgeon (Acipenser schrenckii)

The cryopreservation of sperm was investigated in Amur sturgeon(Acipenser schrenckii) by extendersⅢ.The results showed that the Amour sturgeon semen was preserved in the extender Ⅲ containing Tri-Glu-KCl supplemented with 8% methanol for 5 h,and thawed by 2 step freezing procedure and at 38 ℃ water bath,with motility of(52.3±3.5)%.The fertilization rate of 68.6% and hatching rate of 52.9% were obtained by the fertilization of the preserved sperm activated by well water and stimulator D(10 mmol/L Tris+10 mmol/L NaCl+25 mmol/L Glu,pH 8.0).The low K+ concentration was found in favor cryopreservation of sperm in Amur sturgeon within the concentration ranging from 1 mmol/L to 2 mmol/L.On the contrary,the higher osmotic extender was beneficial to preserve the sperm with extender content from 52 mosmol/kg to 82 mosmol/kg.Moreover,the method of activation was an important factor affecting fertilization rate;hatching rate.