| 一株海洋红酵母的鉴定及其培养基的优化 |
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| 引用本文： | 周鲜娇,田丽,梁如璞,邹文达. 一株海洋红酵母的鉴定及其培养基的优化[J]. 水产科学, 2012, 31(2): 107-110 |
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| 作者姓名： | 周鲜娇 田丽 梁如璞 邹文达 |
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| 作者单位： | 湛江师范学院生命科学与技术学院,广东湛江,524048 |
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| 基金项目： | 湛江市科技攻关计划项目，湛江师范学院自然科学研究项目 |
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| 摘 要： | 通过菌落形态、培养特征和生理生化特征的测定,初步确定海洋红酵母HN-3为红酵母属的胶红酵母。比较不同的碳源、氮源、酵母膏质量分数和海水盐度对该酵母生长的影响,优化其培养基。试验结果表明,最佳培养基为2%葡萄糖、1.5%蛋白胨、1%酵母膏,盐度30。在该培养条件下,酵母菌HN-3的产菌量达到4.80×107cfu/mL,比初始发酵条件下的产菌量(4.20×107cfu/mL)提高了14.3%。
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| 关 键 词： | 鉴定 海洋红酵母 培养基 优化。 |
Identification and Culture Medium Optimization for a Marine Rhodotorula |
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| ZHOU Xian-jiao , TIAN Li , LIANG Ru-pu , ZHOU Wen-da. Identification and Culture Medium Optimization for a Marine Rhodotorula[J]. Fisheries Science, 2012, 31(2): 107-110 |
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| Authors: | ZHOU Xian-jiao TIAN Li LIANG Ru-pu ZHOU Wen-da |
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| Affiliation: | ZHOU Xian-jiao,TIAN Li,LIANG Ru-pu,ZHOU Wen-da( College of Life Science and Technology,Zhanjiang Normal University,Zhanjiang 524048,China ) |
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| Abstract: | The characteristics of colony morphology,culture,physiology and biochemistry were studied in a yeast named HN-3,resembled to Rhoclotorula mucilaginosa.The effects of different carbon,and nitrogen resources and salinity of sea water on the growth of the yeast were studied and the culture medium of the yeast was optimized.The results showed that the best culture medium of the yeast was comprised of 2% glucose,1.5% peptone,1% yeast extract,and sea water with a salinity of 30 for production of the yeast,with 4.80×107cfu/mL,14.3% higher than the initial culture conditions(4.20×107cfu/mL). |
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| Keywords: | identification marine Rhodotorulaum medium optimization |
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