西瓜种质资源遗传多样性的SRAP分析

【研究目的】研究西瓜材料之间的亲缘关系及其分类，为进一步利用和创新材料、培育新的品种提供依据；【方法】采用SRAP技术对64份西瓜种质资源的遗传多样性进行了研究；【结果】从700多个引物组合中筛选出了51个条带清晰、多态性高的引物组合来分析供试材料，共产生431条扩增带，其中243条带具有多态性，多态性频率平均为56.4%，除去非洲野生型57号材料，其它材料间多态率为39.4%。将243条多态性SRAP条带，利用NTSYS软件计算了64个材料间的遗传相似系数，其变化范围为0.47~0.97，除非洲野生型材料57号等4个差异较大的材料，其它材料间变化范围仅为0.87~0.97；【结论】说明目前西瓜育种的大多数材料间同源性较高，遗传分化较小，其遗传基础非常狭窄。该研究结果对利用特殊种质、合理选择杂交亲本具有一定的参考价值。

SRAP Analysis for the Genetic Diversity ofWatermelon Varieties Resources

【OBJECTIVE】Genetic relationships and classifications of a set of watermelon accessions were analyzed to provide the experimental support for utilizing effectively genetic materials for variety development.【METHOD】SRAP technique was adopted to analyze 64 watermelon accessions；【RESULTS】fifty-one primer combinations with clear band pattern and polymorphism were selected from over 700 primer combinations, These primer combinations produced 431 bands in which there were 243 polymorphic bands and the average frequency of the polymorphisms is 56.4%. When the wild-type material No.57 was removed, the frequency of the polymorphisms of other materials is 39.4%.The genetic similarity coefficient of the 64 accessions were calculated by the NTSYS software using 243 polymorphism SRAP bands. It’s ranged between 0.47 - 0.97. When excluding most variable four accessions including No.57, and range decreased to 0.87-0.97；【CONCLUSION】It showed that the watermelon has a limited genetic differentiation among the breeding lines and broaden genetic diversity is very important for watermelon breeding