Root peroxidases and Rhizobium, Bradyrhizobium nodulation affinities of Phaseolus vulgaris L. and P. acutifolius A. Gray congruity backcross populations |
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Authors: | NO Anderson PD Ascher K Haghighi |
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Institution: | (1) Department of Horticultural Science, University of Minnesota, St. Paul, MN, 55108, U.S.A;(2) Department of Pharmacology and Cell Biophysics, University of Cincinnati, Cincinnati, OH, 45267, U.S.A |
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Abstract: | Four parents P. acutifolius var.acutifolius (A19), P. a.var. latifolius `Serowi' (A9),P. a. var. latifolius(A10), and P. vulgaris `Red Cloud' kidney (V1)] were used to create six interspecific recurrent and congruity backcross pedigrees (V1 × A9, A9 × V1, V1 × A10, A10 × V1, V1 × A19, A19 × V1) for evaluation of Rhizobium, Bradyrhizobium nodulation affinities and root peroxidase banding patterns. Most primary hybrids nodulated with all Rhizobium, Bradyrhizobium strains, while recurrent generations nodulated with strains of the recurrent parent, suggesting a number of independent loci
and random assortment for strain-specific nodulation. A proximity matrix of nodulation phenotypes produced a cluster with
V1, two recurrent and two congruity backcross hybrids with V1 as the cytoplasmic parent; a separate cluster with all primary interspecific and balanced, congruity backcross hybrids. A9 and A10were clustered the farthest away from V1. Root peroxidase zymograms of primary hybrids were the summation of the parents and controlled by independent loci. Advanced
hybrids exhibited new root peroxidases, matching those found for otherPhaseolus spp., including ancestralP. coccineus. A proximity matrix of root peroxidase phenotypes produced a cluster with only A9
3V1F1, a cluster containing only A19
3V1 F2, and a large cluster with all P. acutifoliusparents and hybrids with P. acutifolius as the cytoplasmic parent in balanced hybrids or the majority parent in unbalanced hybrids. Estimation of genetic distances
showed primary, interspecific hybrids to be more closely related with the cytoplasmic parent. Recurrent and congruity backcross
generations varied in genetic distances between each respective parent. This is the first report of congruity backcrossing
creating hybrids with biochemical traits of nonparental species.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | electrophoresis hybrid breakdown incongruity interspecifichybridization |
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