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小盐芥 TsPIP1;1 与 TsTIP1;1 基因增强转基因水稻耐盐性
引用本文:李伟,韩娇,黄升财,何蕊,王冰,程宪国. 小盐芥 TsPIP1;1 与 TsTIP1;1 基因增强转基因水稻耐盐性[J]. 植物营养与肥料学报, 2017, 23(4): 957-963. DOI: 10.11674/zwyf.17063
作者姓名:李伟  韩娇  黄升财  何蕊  王冰  程宪国
作者单位:1.中国农业科学院农业资源与农业区划研究所,北京 100081
基金项目:国家重点研发项目(2016YFC0501203),973项目(2015CB150800)
摘    要:【目的】为更好地了解植物水通道蛋白盐胁迫下的调节作用,对小盐芥质膜内在蛋白TsPIP1;1及液泡膜内在蛋白TsTIP1;1在转基因水稻中的盐胁迫生理响应机制进行探究,旨在为水通道蛋白在耐盐作物分子改良育种中的应用提供理论支撑。【方法】以野生型 (WT) 与 T3 代转 TsPIP1;1 及 TsTIP1;1 基因水稻为材料,进行了水培试验,并设置了 0、100、200 mmol/L NaCl 处理。处理一周后,分别测定水稻的光合参数、株高、生物量、相对含水量、失水率及钾、钠含量。【结果】在盐胁迫处理下,与野生型相比,转基因水稻的生物量和含水量明显增加,渗透势和失水率显著降低。转 TsPIP1;1 及 TsTIP1;1 基因水稻根部及地上部的 Na+ 含量都显著降低,K+ 在转基因株系中的累积显著高于野生型,降低了体内 Na+/K+ 比,并且能够保持更强的净光合速率、气孔导度、蒸腾速率及水分利用效率。在 200 mmol/L NaCl 处理下,与野生型相比,TsTIP-5、TsTIP-7 及 TsPIP-19 的株高分别高出 8.2%、11.6%、4.9%;单株干重分别高出 17.9%、23.9%、16.9%;地上部 Na+/K+ 比分别降低 24.3%、24.4%、24.8%;根部 Na+/K+ 比分别降低 29.6%、27.5%、32.4%;渗透势分别显著降低了 18.3%、19.4%、30.3%;相对含水量分别增加了 5.8%、5.5%、5.4%;净光合速率分别增加了50.4%、 78.5%、56.2%。【结论】TsPIP1;1 及 TsTIP1;1 增强了转基因水稻的光合呼吸作用,通过降低植物体内 Na+/K+ 比,参与植物细胞的渗透调节,提高了细胞持水能力,促进转基因水稻的生长发育,增强了水稻的耐盐性。

关 键 词:转基因水稻   盐胁迫   水通道蛋白   渗透调节
收稿时间:2017-02-27

Thellungiella salsuginea TsPIP1;1 and TsTIP1;1 enhanced the salt tolerance of transgenic rice
LI Wei,HAN Jiao,HUANG Sheng-cai,HE Rui,WANG Bing,CHENG Xian-guo. Thellungiella salsuginea TsPIP1;1 and TsTIP1;1 enhanced the salt tolerance of transgenic rice[J]. Plant Nutrition and Fertilizer Science, 2017, 23(4): 957-963. DOI: 10.11674/zwyf.17063
Authors:LI Wei  HAN Jiao  HUANG Sheng-cai  HE Rui  WANG Bing  CHENG Xian-guo
Affiliation:1.Key Laboratory of Plant Nutrition and Fertilizer, Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, China
Abstract:[Objectives] To better understand the regulatory roles of aquaporins in plants in response to salt stress and provide a theoretical support for the application of aquaporin genes in molecular breeding of salt tolerant crop,two aquaporins of Thellungiella salsuginea,plasma membrane intrinsic protein TsPIP1;1 and tonoplast intrinsic protein TsTIP1;1,were investigated by profiling the physiological responses in the transgenic rice under salt stress.[Methods] Wild type (WT) and T3 generations of the transgenic rice carrying the TsPIP1;1 and TsTIP1;1 were subjected to hydroponic solution with 0,100 and 200 mmol/L NaCl in greenhouse,respectively.After one-week's salt treatments,some important physiological parameters such as the photosynthetic rates,plant height,biomass,relative water contents,water loss rates and the contents of Na+ and K+ were determined.[Results] Under 200 mmol/L NaC1 treatment,compared with the wild type,the transgenie rice exhibited higher biomass and relative water contents,and lower water loss rates and osmotic potentials,and demonstrated lower contents ofNa+ and higher contents ofK+ in roots and shoots and thus leading to lower Na+/K+ ratios.The transgenic rice maintained stronger net photosynthetic rate,stomatal conductance,transpiration rate and water use efficiency.Compared with the wild type,in 200 mmol/L NaC1 treatment,the shoot heights in the transgenic lines TsTIP-5,TsTIP-7 and TsPIP-19 were respectively increased by 8.2%,11.6% and 4.9%,and plant dry weights were increased by 17.9%,23.9% and 16.9%,while the Na+/K+ ratios of shoots were reduced by 24.3%,24.4% and 24.8%,and the Na+/K+ ratios of roots were decreased by 29.6%,27.5% and 32.4%.The osmotic potentials were significantly depressed by 18.3%,19.4% and 30.3%,the relative water contents were increased by 5.8%,5.5% and 5.4%,and the net photosynthetic rates were increased by 50.4%,78.8% and 56.2%.[Conclusions] The transgenic rice of carrying the TsPIP1;1 and TsTIP1;1 genes had better growth development,and enhanced the salt tolerance by increasing the photosynthesis and respiration,decreasing the Na+/K+ ratio,participating in cell osmotic adjustment and improving the water holding capacity in plant cells.
Keywords:transgenic rice  salt stress  aquaporin  osmotic adjustment
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