| 家蚕胚胎细胞系(BmE-SWU2)同工酶的研究 |
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| 引用本文： | 关国平,潘敏慧,陈敏,鲁成.家蚕胚胎细胞系(BmE-SWU2)同工酶的研究[J].蚕业科学,2006,32(1):110-113. |
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| 作者姓名： | 关国平 潘敏慧 陈敏 鲁成 |
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| 作者单位： | 西南农业大学蚕学与生物技术学院,农业部蚕桑学重点开放实验室,重庆,400716;西南农业大学蚕学与生物技术学院,农业部蚕桑学重点开放实验室,重庆,400716;西南农业大学蚕学与生物技术学院,农业部蚕桑学重点开放实验室,重庆,400716;西南农业大学蚕学与生物技术学院,农业部蚕桑学重点开放实验室,重庆,400716 |
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| 基金项目： | 国家重点基础研究发展计划“973”项目(编号2005CB-121000) |
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| 摘 要： | 以新建的家蚕胚胎细胞系(BmE-SWU2)细胞为材料,应用非变性聚丙烯酰胺凝胶电泳(Active-PAGE)不连续系统,进行α-酯酶(α-EST)、β-酯酶(β-EST)、乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH)的同工酶研究,观察了在建系过程中同工酶的表达情况,比较了BmE-SWU2细胞系与其源胚胎的同工酶差异。结果显示,在第10代和第25代之间,尚未有明显的表达差异。该细胞系和源胚胎同工酶的比较发现,二者的同工酶表达谱具有很高的相似性。
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| 关 键 词： | 家蚕胚胎细胞系 同工酶 酯酶 乳酸脱氢酶 苹果酸脱氢酶 |
| 文章编号： | 0257-4799（2006）01-0110-04 |
| 收稿时间： | 2005-06-20 |
| 修稿时间： | 2005年6月20日 |
Studies on Isoenzymes of BmE-SWU2 Cell Line Derived From Silkworm Embryo |
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| GUAN Guo-Ping,PAN Min-Hui,CHEN Min,LU Cheng.Studies on Isoenzymes of BmE-SWU2 Cell Line Derived From Silkworm Embryo[J].Acta Sericologica Sinica,2006,32(1):110-113. |
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| Authors: | GUAN Guo-Ping PAN Min-Hui CHEN Min LU Cheng |
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| Institution: | The key Sericultural Laboratory of Agricultural Ministry, College of Sericulture and Biotechnology, Southwest Agricultural Univesity , Chongqing 400 716 , China |
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| Abstract: | To be acquainted with the biochemical characters of isoenzymes in BmE-SWU2 cell line and observe the primary expression of the isoenzyme genes,we carried out the experiments on 4 isoenzymes,viz α-eastase(α-EST),β-eastase(β-EST),lactic dehydrogenase(LDH)and malic dehydrogenase(MDH),in BmE-SWU2,Sf21,Hi5,Sf9 and BmN cell lines by active-PAGE.The differences of isoenzymes between BmE-SWU2 and its original embryos were also compared.The results indicated that there were no obvious differences in isoenzymes between the 10~(th) generation.The 25~(th) generation.The isoenzymes expression pattern between BmE-SWU2 and the embryos showed high similarity. |
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| Keywords: | Bm-SWU2 Isoenzymes Eastase Lactic dehydrogenase Malic dehydrogenase |
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