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大豆DNA导入玉米后代植株蛋白质和油分的分析(英文)
引用本文:张慧英,田夏红,冯锐,刘爱花. 大豆DNA导入玉米后代植株蛋白质和油分的分析(英文)[J]. 农业科学与技术, 2008, 9(6): 74-77
作者姓名:张慧英  田夏红  冯锐  刘爱花
作者单位:张慧英,田夏红,ZHANG Hui-ying,TIAN Xia-hong(广西大学农学院,广西南宁,530005);冯锐,FENG Rui(广西农业科学院,广西南宁,530007);刘爱花,LIU Ai-hua(广西农业职业技术学院,广西南宁,530007) 
基金项目:广西自然科学基金,广西教育厅资助项目 
摘    要:[Objective] The experiment was aimed to explore character variation between different families after DNA introduction and select variant plants with good stability. [Method] The method of pollen-tube-pathway was used to introduce total DNA of soybean into normal maize inbred line 7313 for selecting generation by generation. When field characters of maize, grain colors, grain traits and panicle axis colors were stable, the crude protein, gliadin, glutelin and oil content of grains which were selected from variant strains were detected and compared. [Result] The grain crude protein, gliadin, glutelin and oil content of line 26h-4-3 were significantly different from these of control treatment. The increments of D3 and D4 generation were 10.34% and 26.70%, 6.58% and 6.28%, 15.09% and 70.34%, 55.82% and 51.52% respectively. All indexes of line 26h-3-1 were also higher than these of control treatment and the increments of D3 and D4 generation were 5.67% and 21.63%,1.91% and 2.31%, 10.85% and 62.27%,22.49% and 9.67%. [Conclusion] The crude protein, gliadin, glutelin and oil content of variant line 26h-4-3 and 26h-3-1 were stable, so variant line 26h-4-3 and 26h-3-1 were excellent variant strains which satisfied the requirement of high protein breeding.

关 键 词:Soybean  DNA  Method  of  pollen-tube-pathway  Maize  inbred  line  Protein  Oil

Analyses of Protein and Oil of Maize Offsprings Plants Introducing Soybean DNA
ZHANG Hui-ying,TIAN Xia-hong,FENG Rui,LIU Ai-hua. Analyses of Protein and Oil of Maize Offsprings Plants Introducing Soybean DNA[J]. Agricultural Science & Technology, 2008, 9(6): 74-77
Authors:ZHANG Hui-ying  TIAN Xia-hong  FENG Rui  LIU Ai-hua
Affiliation:1.College of Agriculture, Guangxi University, Nanning 530005; 2. Guangxi Academy of Agricultural Sciences, Nanning 530007;3. Guangxi Agricultural College of Vocational Technology, Nanning 530007
Abstract:[Objective] The experiment was aimed to explore character variation between different families after DNA introduction and select variant plants with good stability. [Method] The method of pollen-tube-pathway was used to introduce total DNA of soybean into normal maize inbred line 7313 for selecting generation by generation. When field characters of maize, grain colors, grain traits and panicle axis colors were stable, the crude protein, gliadin, glutelin and oil content of grains which were selected from variant strains were detected and compared. [Result] The grain crude protein, gliadin, glutelin and oil content of line 26h-4-3 were significantly different from these of control treatment. The increments of D3 and D4 generation were 10.34% and 26.70%, 6.58% and 6.28%, 15.09% and 70.34%, 55.82% and 51.52% respectively. All indexes of line 26h-3-1 were also higher than these of control treatment and the increments of D3 and D4 generation were 5.67% and 21.63%,1.91% and 2.31%, 10.85% and 62.27%,22.49% and 9.67%. [Conclusion] The crude protein, gliadin, glutelin and oil content of variant line 26h-4-3 and 26h-3-1 were stable, so variant line 26h-4-3 and 26h-3-1 were excellent variant strains which satisfied the requirement of high protein breeding.
Keywords:Soybean DNA  Method of pollen-tube-pathway  Maize inbred line  Protein  Oil
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