水稻纹枯病菌拮抗菌B34分离鉴定及杀菌蛋白的获得

从水稻植株上分离到1株对水稻纹枯病菌有较好拮抗作用编号为B34的菌株，室内对水稻纹枯病菌的抑菌带达到10.3 mm。经生理、生化检测及细胞壁化学成分分析，确定其为致黄假单胞菌（Pseudomonas aureofaciens）。其代谢粗提物经蛋白酶作用，拮抗作用消失。经70%饱和度的（NH4）2SO4沉淀，透析后得到粗杀菌蛋白，分别上样SephadexG-100和DEAE纤维素32LKB柱层析系统，分离纯化了杀菌蛋白，经SDS-PAGE检测其分子量约为33.0 kDa，经低温、真空干燥，得到了该杀菌蛋白的结晶体。

Screening,Taxonomy of Antagonistic Strain B34 Against Rhizoctonia solani and Acquirement of the Antiseptic Protein

A strain B34 against Rhizoctonia solani was screened from rice plant,in vitro the inhibition zone width was 10 3 mm. Results of analysis on chemical components in cell wall, physiological and biochemical characters of B34, indicated it was Pseudomonas aureofaciens. When its crude extracts were treated by proteinase K and trypsin, the antagonism disappeared. The crude protein of B34 was obtained with 70% staturated (NH₄)₂SO₄. The protein was purified by ion-exchange and gel filtration LKB chromatography. One antagonistic peak was attained and showed only single band with 33.0 kDa by SDS-PAGE. The purified protein crystal of antiseptic protein was obtained.