桃脂氧合酶(LOX)基因家族cDNA全长克隆与序列分析

根据脂氧合酶保守序列设计的简并引物从桃（瑞蟠5号）果肉总RNA中扩增出795bp的脂氧合酶（lipoxygenase，LOX）基因的保守序列，并结合GeneBank中公开的LOX基因的EST序列经Blast比对分析、序列组装之后设计基因特异引物（GSP）。利用RACE技术共获得3条不同的桃LOX基因cDNA全长序列，分别命名为PpLox1-3。序列分析结果表明，这3个桃LOX基因家族成员与其他植物的LOX基因尽管在核酸水平上的差异比较大，但在蛋白水平上却有较高的同源性。通过对桃LOX基因蛋白序列和其他植物LOX进行系统进化树分析，发现PpLox1属于13-LOX类型，PpLox2-3属于9-LOX类型。

Cloning and analysis of full-length LOX gene family cDNA

The conservative region of Lipoxygenase（LOX）gene from peach(Ruipan 5) was amplified from fruit cDNA, which was combined with the ESTs of LOX genes from GeneBank, and design the GSP primers after Blast aligning and sequence install. Three full-length different cDNA sequences were obtained, which were named PpLox1-3. The result of analysis indicated the three members of peach LOX gene family have high homogeneous with other plant LOX genes in protein level, but high heterogeneous in nucleotide level. Phylogenetic tree based on LOXs in Peach and other species indicated that PpLox1 was belonged to sort of 13-LOX and PpLox2-3 were belonged to sort of 9-LOX.