Effect of methisoprinol on virus replication in cell cultures

The effect of Methisoprinol (active substance: isoprinozine) on the replication of two animal viruses, the TK900 strain of Aujeszky's disease virus and the Roakin strain of the Newcastle disease virus was investigated. When the maximal tolerable doses of the drug were added to two cell cultures (CECC and GMK), its effect on the level of infectious titres of theviruses and their adsorption were assayed. Investigations were also performed to assess the direct effect of Methisoprinol on the viral strains used. The final stage of the experiment aimed at analysing of the replication dynamics of the viruses in the presence of Methisoprinol. Methisoprinol showed no direct effect on the viruses used in the study. Nor did it affect their adsorption. The preparation applied to the culture 24 hours before infection did not influence the replication of viruses, but administered simultaneously with the infection significantly lowered the final titres of viruses. The highest inhibitory effect of the drug was observed during the analysis of the replication dynamics of both viruses in CECC and of pseudorabies virus in GMK cell culture upon the application of the maximal tolerable doses of Methisoprinol and low infectious doses of the viruses.