BHL等4种药物对油菜杀雄效果的研究

采用不同浓度的BHL、快灭灵、乙烯利、二甲四氯钠在油菜蕾薹期对白菜型油菜自交系143C和甘蓝型油菜细胞质雄性不育系203A进行杀雄效果试验。结果表明:用0.5μg/mL BHL在油菜薹高15~20 cm,最大花蕾1.5~2.5 mm时期叶面喷施,单株受药量15~17 mL,间隔10 d后用相同浓度再喷一次,单株受药量8~10 mL,可诱发白菜型油菜91%全不育株率,可使甘蓝型油菜细胞质雄性不育系完全不育,低温不再产生微粉。而另3种药物在本试验中几乎没有杀雄效果。细胞学观察化学杀雄后白菜型油菜143C花药的发育过程:在造孢期造孢细胞粘连,空泡化;减数分裂至四分体期,花药不发育,或绒毡层与花粉母细胞粘连变形;单胞、二三胞花粉期药室有少量畸形花粉粒、或死花粉粒、或完全无花粉粒。

Male Sterilizing Effect of Four Drugs, eg. BHL on Rapeseed

Plants of Brassicacampestris breeding line 143C and Brassica uapus line CMS 203A were treated with various concentrations of BHL,carf entrazo ne-ethy,ethephon and MCPA-Na to detect their male sterilization.The result indicated that 0.5 ug/mL BHL,first sprayed with 15~17mL per plant in peduncle-growing period with 1.5~2.5 mm long bud and 10 days later done again with 8~10 mL per one,induced about 91% complete sterile plants on Brassica campestris breeding line 143C and 100% controlling trace amount pollens on B rassica uapus line CMS 203A,making full sterility at low temperature.However,other three drugs barely caused male sterility in this experiment.Microscopic observations on aborted anther development of B rassica campestris breeding line 143C induced by BHL showed that sporogenous cells adhered to each other and inflated like vacuolization at the stage of sporogenous cell;anther did not grow or tapetum and microspore mother cells adhered to mutually and distorted at the stage of meiosis and tetrad;little abnormal pollen existed or dead pollen or no pollen at the stage of mononucleus microspore and mature period.