激素对陆地棉和海岛棉茎尖培养的影响

1AA,IBA,NAA,2,4-D对陆地棉和海岛棉茎尖培养有一定的促进作用,BA,KT,ZT则抑制棉花茎尖培养,在一定激素浓度范围内,随着激素浓度的升高,对棉花茎尖培养过程中地上部和地下部的生长均有促进作用,但继续增加激素浓度,虽对棉花茎尖培养不定根的形成仍有促进作用,但却抑制了地上部的生长.适合棉花茎尖培养的最佳激素和浓度为0.01～0.1 mg/L IBA.其中,在含有0.1 mg/LIBA的培养基中,陆地棉和海岛棉基因型间茎尖培养的效果存在着显著差异.以海岛棉不定根的形成能力较陆地棉容易,而地上部的长势则陆地棉优于海岛棉.其中,在陆地棉中又以浙506茎尖培养效果较好;泗棉3号相对较差.在海岛棉中,比马3-33茎尖培养效果较好,而米奴非10的茎尖培养效果较差.

The effect of plant hormone on the shoot tip culture of Gossypium hirsutum and G. barbadense.

Plant hormone IAA, IBA,NAA and 2,4 D benefited to the shoot tip culture of Gossypium hirsutum and G barbadense , while plant hormone BA,KT and ZT suppressed the shoot tip culture In a range of 0 01 0 1 mg/L IBA, the medium promoted to the shoot tip culture, and the concentration being more than 0 1 mg/L, the medium promoted the formation of adventitious root, but it suppressed the differentiation of axial bud and the shoot tip growth The best type and concentration of plant hormone was IBA in the concentration of 0 01 0 1 mg/L There was significant difference among the genotypes of G hirsutum and G barbadense in the shoot tip culture with the medium of 0 1 mg/L IBA G barbadense was better than G hirsutum in the formation of rooting, but G hirsutum was better in the growth of regenerated plant than G barbadense In G hirsutum , Zhe506 was the best, Simian3 was bad In G barbadense , Pima3 33 was the best, Menoufi10 was bad