| 超声波辅助酶促猪油甘油解制备甘油单酯的研究 |
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| 引用本文： | 赵哲,张丹妮,高纯阳,夏元芳,魏微,赵海珍,陆兆新. 超声波辅助酶促猪油甘油解制备甘油单酯的研究[J]. 江西农业学报, 2013, 0(12): 101-105 |
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| 作者姓名： | 赵哲 张丹妮 高纯阳 夏元芳 魏微 赵海珍 陆兆新 |
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| 作者单位： | 南京农业大学食品科技学院,江苏南京210095 |
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| 基金项目： | 国家级大学生创新创业训练计划项目（201110307041）;南京农业大学大学生科研训练项目（1118A03）. |
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| 摘 要： | 以猪油和丙三醇为原料,采用超声辅助酶促甘油解反应合成甘油单酯,通过单因素试验及正交试验,确定其最佳酶促合成条件。结果表明,最佳反应条件:叔戊醇为溶剂,Novozym 435用量10%(猪油质量分数),反应温度45℃,甘油与猪油物质的量的比4∶1,反应时间6 h,超声波频率28 kHz,超声功率250 W。在此条件下,甘油单酯产率达到(84.76±1.96)%。超声辅助酶促反应,不仅可以提高产物产率,还可缩短反应时间。
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| 关 键 词： | 脂肪酶 甘油单酯 猪油 超声波 甘油解 |
Study on Ultrasound -assisted Synthesis of Monoglyceride by Lipase -catalyzed Glycerolysis of Lard with Glycerol |
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| Affiliation: | ZHAO Zhe, ZHANG Dan - ni, GAO Chun - yang, XIA Yuan - fang, WEI Wei, ZHAO Hal - zhen , LU Zhao - xin ( College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China) |
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| Abstract: | Monoglyceride was synthesized by lipase -catalyzed glycerolysis of lard with glycerol through ultrasound assistance. Single - factor experiment and orthogonal test were conducted to optimize the glycerolysis conditions of lard. The optimum conditions for monoglyceride synthesis were acquired as follows： tert - amyl alcohol as reaction medium, Novozym 435 lipase dosage 10% （ calculated on the basis of the mass of lard）, reaction temperature 45 ~C, molar ratio of glycerol to lard 4： 1, reaction time 6 h, ul- trasonic frequency 28 kHz, and ultrasonic power 250 W. Under these optimum conditions, the productive rate of monoglyceride was up to 84.76% -＋ 1.96%. The ultrasound - assisted lipase - catalyzed treatment could not only improve the yield of product, but also shorten the reaction time. |
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| Keywords: | Lipase Monoglyceride Lard Ultrasonic treatment Glycerolysis |
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