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| 猪血免疫球蛋白G提取及分子量测定 | |
| 引用本文： | 孙高超,孙圣君,刘高升,张娜,毕可东.猪血免疫球蛋白G提取及分子量测定[J].安徽农业科学,2009,37(23):11017-11018. |
| 作者姓名： | 孙高超 孙圣君 刘高升 张娜 毕可东 |
| 作者单位： | 1. 青岛农业大学,山东青岛,266109 2. 山东省乳山市畜牧局,山东威海,264500 3. 吉林大学,吉林长春,130062 |
| 摘 要： | 目的]纯化猪血免疫球蛋白IgG，并测定其轻链与重链的分子量。方法]采用分步硫酸铵盐析法粗提猪血浆中的免疫球蛋白G，透析袋脱盐后用DEAE-SephadexAS0对IgG进行纯化，对纯化后的免疫球蛋白进行SDS-PAGE电泳，采用AlphaEaseFC凝胶分析软件对凝胶图像进行分析。结果]3份样品中免疫球蛋白的浓度分别为2．712、2．679、2．489mg／ml；电泳后每泳道均出现2条蛋白带，说明所提免疫球蛋白的纯度较高；2条蛋白带分别为IgG的重链（H链）与轻链（L链），与标准蛋白Marker进行比较可知，H链的分子量约为57KDa，L链的分子量约为26KDa。结论]得到了高纯度的猪IgG，并进一步确定了IgG轻链与重链的分子量。 |
| 关 键 词： | 猪血浆 免疫球蛋白 分子量 |
Extraction of Immunoglobulin G from Pig Blood and Determination on Its Molecular Weight |
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| SUN Gao-chao et al.Extraction of Immunoglobulin G from Pig Blood and Determination on Its Molecular Weight[J].Journal of Anhui Agricultural Sciences,2009,37(23):11017-11018. | |
| Authors: | SUN Gao-chao |
| Institution: | SUN Gao-chao et al(Qingdao Agricultural Unverisity,Qingdao,Sh,ong 266109) |
| Abstract: | Objective] The study was to purify the immunoglobulin IgG from pig blood and determine the molecular weight of its light and heavy chains.Method] Immunoglobulin G from pig plasma was extracted by sequential ammonium sulfate precipitation method,and the IgG was purified by DEAE-SephadexA50 after desalination treatment with dialysis bag,the purified immunoglobulin was taken for SDS-PAGE electrophoresis,and the gel image was analyzed with AlphaEaseFC analysis software.Result] Immunoglobulin concns.of 3 samp... |
| Keywords: | Pig plasma Immunoglobulin Molecular weight |
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