Analysis of Short-Term Cortisol Stress Response in Channel Catfish by Anesthetization with Metomidate Hydrochloride and Tricaine Methanesulfonate |
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| Authors: | M S Smith N J Booth W S Stephens C A Goudie B A Simco |
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| Institution: | 1. Ecological Research Center, University of Memphis, Building 8, South Campus, Memphis, Tennessee 38152, USA;2. Agricultural Research Service, Warmwater Aquaculture Research Unit, Post Office Box 38, Stoneville, Mississippi 38776, USA |
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| Abstract: | The ability of the anesthetics metomidate hydrochloride and tricaine methanesulfonate (MS-222) to mitigate the cortisol stress response of Channel Catfish Ictalurus punctatus was evaluated during a 10-min confinement stress. The cortisol concentrations of Channel Catfish anesthetized in metomidate hydrochloride remained consistent throughout the 10-min exposure; however, for fish anesthetized with MS-222 and nonanesthetized fish, cortisol concentrations were approximately 7- and 22-fold higher, respectively, than the baseline concentrations. While both anesthetics reduced cortisol concentrations relative to those of nonanesthetized fish, these results suggest that MS-222 is an appropriate anesthetic to use during the initial 5 min of sedation and that metomidate hydrochloride is appropriate for longer periods of sedation. Received November 18, 2014; accepted April 9, 2015 |
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