Growth Inhibition of Bacterial Fish Pathogens and Quorum-Sensing Blocking by Bacteria Recovered from Chilean Salmonid Farms |
| |
| Authors: | Mery de la Fuente Paz Jopia Gerardo González-Rocha Nicolás Guiliani Katherine Sossa |
| |
| Affiliation: | 1. Laboratorio de Biotecnología e Ingeniería Acuícola, Departamento de Ingeniería Ambiental y Recursos Naturales, Facultad de Ingeniería, Universidad Católica de la Santísima Concepción, Alonso de Ribera 2850, Concepción, Chile;2. Departamento de Ciencias Biológicas y Químicas, Facultad de Ciencias, Universidad San Sebastián, Concepción, Chile;3. Laboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, Chile;4. Laboratorio de Antibióticos, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile;5. Laboratorio de Comunicación Bacteriana, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile |
| |
| Abstract: | The main goal of this study was to find bacterial isolates with the ability to inhibit the growth of the fish pathogens Aeromonas hydrophila, Vibrio anguillarum, and Flavobacterium psychrophilum and to inhibit the blockage of the quorum-sensing (QS) system. A total of 80 gram-negative strains isolated from various freshwater Chilean salmonid farms were studied. We determined that 10 strains belonging to the genus Pseudomonas inhibited at least one of the assayed fish pathogens. Of these, nine strains were able to produce siderophores and two strains were able to inhibit the growth of all assayed pathogenic species. When the 80 strains were examined for QS-blocking activity, only the strains Pseudomonas sp. FF16 and Raoultella planticola R5B1 were identified as QS blockers. When the QS-blocker strains were analyzed for their ability to produce homoserine lactone (HSL) molecules, thin-layer chromatography analysis showed that both strains were able to produce C6-HSL– and C8-HSL–type molecules. Strain R5B1 did not show growth inhibition properties, but strain FF16 also led to inhibition of growth in A. hydrophila and F. psychrophilum as well as to siderophore production. Pseudomonas sp. FF16 exhibited potentially useful antagonistic properties and could be a probiotic candidate for the salmon farming industry. Received July 31, 2014; accepted December 17, 2014 |
| |
| Keywords: | |
|
|
