| 三唑磷对麦穗鱼脑组织中乙酰胆碱酯酶的诱导 |
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| 引用本文： | 李少南,谢显传,谭亚军,朱国念.三唑磷对麦穗鱼脑组织中乙酰胆碱酯酶的诱导[J].农药学学报,2005,7(1):59-62. |
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| 作者姓名： | 李少南 谢显传 谭亚军 朱国念 |
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| 作者单位： | 浙江大学,农药与环境毒理研究所,浙江,杭州,310029;上海市农业科学研究院,植物保护研究所,上海 201106 |
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| 基金项目： | 浙江省自然科学基金(No. 392043). |
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| 摘 要： | 为了检测有机磷杀虫剂对鱼脑组织中乙酰胆碱酯酶(AChE, EC 3.1.1.7)的诱导,采用间接非竞争酶联免疫吸附测定法(ELISA)检测了三唑磷胁迫下麦穗鱼Pseudorasbora parva脑组织中乙酰胆碱酯酶含量的变化。当暴露浓度仅为0.1 μg /L时,三唑磷即对AChE产生诱导。由于存在诱导效应,三唑磷对酶活性的抑制在一定程度上被酶蛋白含量的提高所掩盖。研究结果表明,以酶蛋白替代总蛋白来衡量粗酶液中AChE的活性,可以明显提高对有机磷胁迫作用的检测灵敏度。
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| 关 键 词： | 麦穗鱼 乙酰胆碱酯酶 三唑磷 ELISA |
| 文章编号： | 1008-7303(2005)01-0059-04 |
| 收稿时间： | 2004/1/13 0:00:00 |
| 修稿时间： | 2004年1月13日 |
Induction of Triazophos to Brain Acetylcholinesterase from Topmouth Gudgeon,Pseudorasbora parva |
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| LI Shao-nan,XIE Xian-chuan,TAN Ya-jun and ZHU Guo-nian.Induction of Triazophos to Brain Acetylcholinesterase from Topmouth Gudgeon,Pseudorasbora parva [J].Chinese Journal of Pesticide Science,2005,7(1):59-62. |
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| Authors: | LI Shao-nan XIE Xian-chuan TAN Ya-jun and ZHU Guo-nian |
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| Institution: | 1.Institute of Pesticides and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China2.Institute of Plant Protection, Shanghai Academy of Agricultural Science, Shanghai 201106, China |
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| Abstract: | For the purpose of detecting of induction of acetylcholinesterase (AChE, EC 3.1.1.7) by organophoshorous insecticides, amount of brain AChE of a freshwater fish, i.e., topmouth gudgeon , Pseudorasbora parva, which underwent exposure of triazophos, was detected using an indirect non-competitive ELISA. The in vivo AChE was induced at such a low concentration of 0.1 μg /L. Due to the induction inhibition on activity of AChE by triazophos was masked, in some extent, by the increase of the enzyme content. Result of the present research indicated that a sensitive detecting of exposure could be achieved if specific activity of AChE was evaluated by the enzyme content, rather than by the total protein content of the crude extract. |
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| Keywords: | Pseudorasbora parva acetylcholinesterase triazophos ELISA |
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