南京不同类型梅花品种香气成分的比较研究

1 材料与方法 采用活体植株动态顶空套袋采集法和TCT／GC／MS（热脱附／气相色谱／质谱联用）技术分析南京梅花山真梅种系直枝梅类宫粉型‘玉露宫粉’、玉蝶型‘宇治里’、黄香型‘黄金鹤’、朱砂型‘姬千鸟’、绿萼型‘变绿萼’、洒金型‘复瓣晚跳’及垂枝梅类白碧垂枝型‘双碧垂枝’的香气组成。2002年3月513采样，将采样后的吸附管套上聚四氟乙烯套，放在干燥器中低温保存。样品分析时间为2002年4月27日。同时采集和分析空气作对照。采用Xcalibur 1．2版本软件及NIST98谱图库进行梅花香气成分的检索，兼顾挥发物出峰的保留时间鉴定。

Comparison of Different Cultivars of Prunus mume''''s Major Gas Ingredients

AIM: To investigate the effect of bone marrow (BM)-derived mesenchymal stem cells (MSCs) on the proliferation of hepatic stellate cells (HSCs) in vitro. METHODS: MSCs and HSCs were isolated from BM and liver respectively in Fischer 344 rats. HSCs were re-cultured on plastic and appeared activated morphologically. The coculture system was set up by transwell insert with pore size 0.4 μm and 6-well plates. The 5th passage HSCs were cultured on the 6-well plastic plate and MSCs or buffalo rat liver cells (BRLs) or HSCs seeded on the transwell insert. Smooth muscle α-actin (α-SMA) and desmin expression of HSCs were tested by immunocytochemistry and quantified by IBAS 2.5 software. RESULTS: After coculture for 24 h, the proliferation of HSCs was inhibited to a moderate extent by MSCs. With the coculture time lasted, the significant growth suppression of HSCs was affected by MSCs (15.7% and 30.3%) but not BRL cells in 48 h and in 72 h. After coculture for 72 h, the expression of α-SMA in HSCs was lower in MSCs coculture system than that with BRLs or HSCs alone system （50.2% vs 90.2%, 95.6%, P＜0.01), while the expression of desmin in HSCs had no difference in three coculture system. CONCLUSION: MSCs can inhibit the proliferation and activation of hepatic stellate cells by secreting some cytokines, which might play a protective role against liver fibrosis.