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The first report on the occurrence race 9 of the tomato leaf mold pathogen Cladosporium fulvum (syn. Passalora fulva) in Cuba |
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| Authors: | Bernal-Cabrera Alexander Martínez-Coca Benedicto Herrera-Isla Lidcay Ynfante-Martínez Danay Peteira-Delgado Belkis Portal Orelvis Leiva-Mora Michel de Wit Pierre J. G. M. |
| Affiliation: | 1.Laboratorio de Fitopatología, Departamento de Agronomía, Facultad de Ciencias Agropecuarias, Universidad Central “Marta Abreu” de Las Villas, Road to Camajuaní km 5.5, 54 830, Santa Clara, Cuba ;2.Department of Plant Protection, Laboratory of Mycology, National Animal and Plant Health Center, Street to Tapaste and National Highway km 22.5, Post office box 10, San José de las Lajas, Havana, Cuba ;3.Departamento de Biología, Facultad de Ciencias Agropecuarias, Universidad Central “Marta Abreu” de Las Villas, Road to Camajuaní km. 5.5, 54 830, Santa Clara, Cuba ;4.Centro de Investigaciones Agropecuarias, Facultad de Ciencias Agropecuarias, Universidad Central “Marta Abreu” de Las Villas, Road to Camajuaní km. 5.5, 54 830, Santa Clara, Cuba ;5.Laboratorio de Biotecnología, Facultad de Ciencias Agropecuarias, Universidad Técnica de Ambato, Cantón Cevallos vía a Quero, sector el Tambo-la Universidad, Cevallos, 1801334, Ambato, Tungurahua, Ecuador ;6.Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands ; |
| Abstract: | Leaf mold caused by the fungus Cladosporium fulvum (syn. Passalora fulva) has been a serious disease in greenhouse tomatoes in Cuba, but less damage is seen in outdoor grown plants. This study was conducted to identify races of the causal agent of tomato leaf mold. A total of 36 single conidial isolates obtained from different provinces in Cuba and the reference strain race 0 were inoculated on a differential set of tomato cultivars carrying different Cf resistance genes. Further, a polymerase chain reaction (PCR) was developed using Avr9 gene-specific primers. As result, all isolates could overcome the Cf-9 resistance gene only, but not resistance genes Cf-2, Cf-4 and Cf-5 and must therefore be coined race 9. Race identity was also confirmed by PCR analysis, which showed that the Avr9 gene was not amplified in any of 36 single conidial isolates studied proving that they all lack this gene. This is the first report of the occurrence of race 9 of C. fulvum in Cuba. Pyramiding other Cf genes in hybrid of tomato may provide a more durable level of resistance to C. fulvum. |
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