新型开放式呼吸测热装置设计及应用试验

[目的]提高小型试验动物呼吸代谢检测的精确度。[方法]结合国际上常用的开放式呼吸测热流程,自主研制小型试验动物自控开放式呼吸测热装置,并应用该装置对Wistar系大白鼠4个不同生长期的耗氧代谢率、能量代谢率进行测定。[结果]雄鼠在生长状态下的耗氧代谢率和能量代谢率随着体重的增加而降低,而在绝食状态下则变化不大。雌鼠生长状态下,耗氧代谢率和能量代谢率随着体重的增加,表现为先增加后降低再增加;而绝食状态下,耗氧代谢率和能量代谢率随着体重的增加,先降低后增加。[结论]该装置技术流程先进、测试指标齐全、重复性好。

Design of Open-respiratory Calorimetry Measuring Device and Its Application in White Rats

[Objective]To improve the detection accuracy of respiratory metabolism in small experimental animals.[Method]After combination of conventional open-respiratory calorimetry device,a novel open-respiratory calorimetry apparatus was designed and was used to determine the metabolic rate for oxygen consumption and energy.[Result]For male rats,the metabolic rates of oxygen consumption and energy decreased with increasing body weight in growth stage,but did not change much in fasting stage.For male rats,the metabo...