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| 虎杖组织培养技术研究 | |
| 引用本文： | 易霭琴,童方平,宋庆安,丁婕,李贵,黄振.虎杖组织培养技术研究[J].湖南林业科技,2007,34(1):10-12. |
| 作者姓名： | 易霭琴 童方平 宋庆安 丁婕 李贵 黄振 |
| 作者单位： | 1. 湖南省林业科学院,湖南,长沙,410004;湖南省林木无性系育种重点实验室,湖南,长沙,410004 2. 湘潭县林业局,湖南,湘潭,411228 3. 中南林业科技大学,湖南,长沙,410004 |
| 摘 要： | 以虎杖带腋芽的茎段为试验材料,研究虎杖组织培养与快速繁殖的适宜条件。试验结果表明,虎杖在MS 6—BA 2.0 mg/L IBA 0.2 mg/L 3%蔗糖 0.7%琼脂培养基上增殖效果最好,培养30 d,增殖倍数达到3.4以上;在1/2MS IBA 0.5 mg/L 3%蔗糖 0.7%琼脂培养基上生根效果最好,生根率100%,平均根长2.0 cm,平均苗高3.5 cm,以圃地表土为基质,在温室大棚内间歇喷雾的移栽成活率达96.25%。 |
| 关 键 词： | 虎杖 组织培养 增殖倍数 移栽 成活率 |
| 文章编号： | 1003-5710（2007）01-0010-03 |
| 收稿时间： | 2006-09-04 |
| 修稿时间： | 2006-11-24 |
The tissue culture technology of Polygonum cuspidatum sieb et zucc |
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| YI Aiqin,TONG Fangping,SONG Qing'an,DING Jie,LI Gui,HUANG Zhen.The tissue culture technology of Polygonum cuspidatum sieb et zucc[J].Hunan Forestry Science & Technology,2007,34(1):10-12. | |
| Authors: | YI Aiqin TONG Fangping SONG Qing'an DING Jie LI Gui HUANG Zhen |
| Abstract: | The tissue culture and suitable the condition of fast reproduction of Polygonum cuspidatum sieb et zucc were studied in the paper based on the experimental material of the axillary buds of it.The test result indicated that: The best multiplies effects was using MS 6-BA 2.0mg/L IBA 0.2 mg/L 3% sucrose 0.7% agar as the culture medium,raises 30 d,the multiplication multiple achieved above 3.4.The best takes roots effects was using 1/2MS IBA 0.5mg/L 3% sucrose 0.7% agar culture medium,takes root to lead 100%,average root long 2.0cm and average seedling height 3.5 cm.Taking the garden surface soil as the matrix,the intermittent atomization transplants the survival rate in the greenhouse awning to reach 96.25%. |
| Keywords: | Polygonum cuspidatum sieb et zucc the tissue culture multiplication transplants survival rate |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |