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| 牙鲆微卫星分子标记与生长性状的相关性分析 | |
| 引用本文： | 王桂兴,刘永新,孙效文,刘海金.牙鲆微卫星分子标记与生长性状的相关性分析[J].东北农业大学学报,2009,40(7). |
| 作者姓名： | 王桂兴 刘永新 孙效文 刘海金 |
| 作者单位： | 1. 大连水产学院生命科学与技术学院,辽宁大连,116023 2. 东北农业大学动物科学技术学院,哈尔滨,150030 3. 中国水产科学研究院黑龙江水产研究所,农业部北方鱼类生物工程育种重点开发实验室,哈尔滨,150070 4. 中国水产科学研究院,北京,100039 |
| 基金项目： | 国家科技支撑计划(2006BAD01A12017);;中国水产科学研究院基本科研业务费专项资金资助项目(2007B004) |
| 摘 要： | 研究利用30对牙鲆微卫星标记,对牙鲆雌核发育家系91个个体基因组DNA进行检测,在30对微卫星标记中,共检测到124个等位基因。其中,各座位的等位基因数3～8个,等位基因片段大小为80～170bp,平均有效等位基因数3.257。其中poli11座位的等位基因数目最多为8。平均多态信息含量为0.6197,平均杂合度为0.6672。利用SAS8.2中的一般线性模型(GLM)对30个微卫星标记与牙鲆的生长性状进行连锁显著性检验。发现在30个微卫星座位中,有8个座位分别与体重、体长、体高显著相关。其中,poli6TUF位点与体重相关;poli30TUF、poli107TUF、poli108TUF、poli116TUF、poli123TUF、poli130TUF6个座位与体长显著相关;poli145TUF与体重、体长显著相关,而与体高相关性不显著。poli9-8TUF与体重、体长、体高3个性状均显著相关(P<0.05)。将基因型与生长性状进行Duncan多重比较,找到4种与生长性状相关的基因型。 |
| 关 键 词： | 牙鲆 微卫星 生长性状 基因型 相关性分析 |
Correlation analysis of microsatellite DNA marks with growth trait of Japanese flounder (Paralichthys olivaceu) |
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| WANG Guixing,LIU Yongxing,SUN Xiaowen,LIU Haijin.Correlation analysis of microsatellite DNA marks with growth trait of Japanese flounder (Paralichthys olivaceu)[J].Journal of Northeast Agricultural University,2009,40(7). | |
| Authors: | WANG Guixing LIU Yongxing SUN Xiaowen LIU Haijin |
| Institution: | 1. College of Aqual-life Sciences and Technology;Dalian Fisheries University;Dalian Liaoning 116023;China;2. College of Animal Sciences Technology;Northeast Agricultural University;Harbin 150030;3. Key Laboratory of Engineering Breeding of North Fish;Ministry of Agriculture;Heilongjiang Fisheries Research Institute;Chinese Academy of Fisheries Sciences;Harbin 150070;4. Chinese Academy of Fisheries Sciences;Beijing 100039;China |
| Abstract: | Total 30 microsatellite DNA markers were selected to analyze the genomic DNA of 91 gynogenesis progenies from the gynogenesis family. The results showed that total 124 different alleles were found,and the number of allele was 3-8 in each locus. The DNA fragment length was 80-170 bp,the number of mean valid alleles was 3.257,the value of mean heterozygosity was 0.6672 and the mean Polymorphism Information Content (PIC) was 06197. The SAS 8.2 was used to analyze the effect of these 30 microsatellites on body ... |
| Keywords: | Paralichthys olivaceu microsatellite marker growth trait genotype correlation analysis |
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