利用分子生物技术对厌氧颗粒污泥中微生物种群结构的研究

利用F ISH、RTQ-PCR和DGGE等分子生物技术对厌氧产甲烷颗粒污泥中微生物种群的多样性、空间分布和定量关系进行研究,并对其中的优势古细菌进行系统发育分析。结果表明:颗粒污泥中真细菌主要分布在颗粒污泥外层,古细菌则主要分布在内层;古细菌含量低于真细菌,但有逐渐增多的趋势;随着反应器有机负荷的增加以及运行时间的延长,真细菌种群结构相对稳定,而古细菌种群结构则发生了较明显变化,其中占优势的古细菌种类逐渐减少,主要包括甲烷微粒菌(M ethanocorpuscu lum)、甲烷杆菌(M ethanobacter ium)和甲烷髦毛菌(M ethanosaeta)等。

Study on microbial community in anaerobic granular sludge by molecular technology

The biodiversity,spatial distribution and quantification of different microbial species in the anaerobic methanogenic granules were investigated by FISH,RTQ-PCR,DGGE,and the phylogenetic analysis of dominant archaea were conducted.The results indicated that: most eubacteria were located in the surface area of the(granule),while most archaea were located in the inner layer;the quantity of archaea was less than eubacteria,but increased gradually;as the organic loading rate of the reactor increased and the operating time elapsed,the eubacterial community was kept relatively stable,while the archaeal community was changed significantly,which(resulted) in the gradual decrease of the archaeal varieties;in the end of the operation,the dominant species of(archaea) in this methanogenic granule were Methanocorpusculum,Methanobacterium,Methanosaeta,and etc.