球根秋海棠金正日离体再生影响因素研究

为了建立球根秋海棠组培快繁体系,以球根秋海棠（BegoniatuberhybridaVoss）品种金正日的叶为外植体,研究了培养中各因素对叶基部的影响。结果表明：不同叶片部位外植体的诱导和分化能力不同,叶基部愈伤组织诱导和分化能力最强,诱导率和分化率分别为85．7％和85．4％;暗培养和接种前4℃低温预处理降低了外植体的诱导率和分化率,在金正日花离体培养中不宜采用;叶基部最适宜的诱导和分化培养基为MS＋2,4-D1．0mg·L-1＋BA2．0mg·L-1＋NAA0．5mg·L-1。

Influencing Factors of Regeneration in vitro on Begonia tuberhybrida Voss cv Jinzhengri

In order to establish rapid propagation system of tissue culture for Begonia tuberhybrida Voss, the leaves of Begonia tuberhybrida Voss cv Jinzhengri were used as explants for regeneration in ＂vitro ,influencing factors of cultivation in vitro were studied. The results showed that the rates of induction and differentiation of four kinds of explants were different,the leaf base had the highest induction and differentiation rates of 85.7 and 85.4% ;the induction and differentiation rates reduced after dark culturing and in 4% before inoculation, which were not fit to be used in leaf base culture in vitro of Begonia tuberhybrida Voss cv Jinzhengrithe best medium for induction and differentiation of the leaf base was MS＋2,4-D 1.0 mg＇L1 ＋BA 2.0 mg.L-1 ＋NAA 0.5 mg-I.-1.