高能混合粒子场诱发的小麦矮杆突变体的SSR分析

为了研究高能混合粒子场(CR)和γ射线两种不同处理方法诱变小麦产生的具有相同表型的突变体之间的分子差异,选用随机分布于小麦21对染色体上的114对微卫星(SSR)引物,对CR和γ射线处理冬小麦品种ZY9和ZH7获得的矮杆突变体M3代进行SSR分析。结果表明,CR处理产生的矮杆突变体的多态性位点主要分布在染色体2A、2B、2D、3D和5A上,而γ射线处理产生的矮杆突变体的多态性位点主要分布在染色体2A、2B、2D、4A和5A上;与γ射线处理相比,CR处理较容易产生扩增条带的增加和扩增条带长度的差异,不易产生扩增条带的缺失。序列分析表明,CR处理产生的变异主要是碱基的置换和插入,其中碱基T为易发生突变的碱基。CR诱变能够在DNA水平上导致小麦遗传物质变异,其诱变机制不同于γ射线,是一种有效的诱发突变新途径。

SSR ANALYSIS OF DWARF MUTANTS DERIVED FROM MIXED PARTICLE FIELD IRRADIATION IN WHEAT

M_3 dwarf mutants derived from both mixed particle field(CR) and γ-rays irradiation in winter wheat varieties ZY9 and ZH7 were used to compare the DNA molecular differences of two induced mutagenesis by SSR polymorphism analysis.114 sets of SSR markers randomly distributed over the 21 chromosomes of wheat were included.The results showed that the SSR polymorphic loci mostly distributed in chromosomes 2A,2B,2D,3D and 5A of the M_3 dwarf mutants induced by CR;but in chromosomes 2A,2B,2D,4A and 5A by γ-rays in wheat varieties ZY9 and ZH7.Compared to the γ-rays irradiation,more DNA fragments and change of molecular weight could be induced in the mutants derived from CR irradiation in ZY9 and ZH7,but less band lack was found in M_3 dwarf mutants derived from CR irradiation.Sequence analysis showed that base substitution and insertion were the main mutation types induced by CR irradiation,and base T might be the hotspot base to mutation.It was concluded that CR irradiation could result in DNA variation of wheat and could be a new effective approach for crop mutation breeding.